Abstract
In this study, we developed an integrated, low-cost microfluidic cell culture system that is easy to use. This system consists of a disposable polystyrene microchip, a polytetrafluoroethylene valve, an air bubble trap, and an indium tin oxide temperature controller. Valve pressure resistance was validated with a manometer to be 3 MPa. The trap protected against bubble contamination. The temperature controller enabled the culture of Macaca mulatta RF/6A 135 vascular endothelial cells, which are difficult to culture in glass microchips, without a CO2 incubator. We determined the optimal coating conditions for these cells and were able to achieve stable, confluent culture within 1 week. This practical system is suitable for low-cost screening and has potential applications as circulatory cell culture systems and research platforms in cell biology.
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This study was partially supported by the A-STEP Seeds Actualization Type program of the Japan Science and Technology Agency (JST).
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Tazawa, H., Sunaoshi, S., Tokeshi, M. et al. An Easy-to-Use Polystyrene Microchip-based Cell Culture System. ANAL. SCI. 32, 349–353 (2016). https://doi.org/10.2116/analsci.32.349
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DOI: https://doi.org/10.2116/analsci.32.349