Abstract
Vaterite is one of three non-hydrate calcium carbonate crystalline polymorphs and is formed as an initial phase under pseudo-biological conditions. However, biological hard tissues that use vaterite are rare; the reason for vaterite rarely appearing in vivo is still unclear. There is consensus that, in phosphate-containing solutions, vaterite barely forms and amorphous calcium carbonate (ACC), the precursor of crystalline calcium carbonate and considered as aggregation of growth unit of vaterite, is stabilized. In this study, to clarify the biomineralization process, we investigated how phosphate acts as an inhibitor of vaterite growth. We measured vaterite growth rates in situ and estimated the essential crystal growth parameter, edge free energy, in the Ca-CO3-PO4 system in relation to the physico-chemical properties of ACC. The effects of PO4 on the ACC structure and dynamics were also observed.
Co-existed PO4 reduced the growth rate of vaterite even when it was added in µM-scale concentrations. The surface free energy of vaterite increased with increasing PO4 concentration and was 10× higher in a 10 μM PO4-containing solution than in a PO4-free solution. Spectroscopic analyses showed that the chemical bonds in ACC particles were drastically changed by the addition of μM-scale PO4, and the particles could no longer transform into vaterite. We conclude that PO4 inhibits vaterite growth and changed the ACC structure. And the original growth units of vaterite were also modified to the other structures. Thus, vaterite crystals could not grow by association of these growth units, which resulted in an increase in the apparent surface free energy of vaterite.
Special collection information can be found at http://www.minsocam.org/MSA/AmMin/special-collections.html.
Acknowledgments
We thank A. Ito and Y. Sogo for their assistance with the optical microscopy experiments. A. Oyane helped with FE-SEM/EDX measurements. We also thank T. Goto for SAXS analysis, N. Sugimura and T. Shibue for Raman and NMR measurements. We thank K. Nakamura and Y. Kimura for their advice. This research was partially funded by the Material Characterization Central Laboratory, Waseda University and a grant-in-aid for doctoral students (DC2) and start-up for young researchers (No. 15H06488) from the Japan Society for the Promotion of Science (JSPS), Ministry of Education, Culture, Sports, Science and Technology (MEXT), Grant No. 25–2283.
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Manuscript handled by Mickey Gunter
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