doiSerbia

The application of HP-GFC chromatographic method for the analysis of oligosaccharides in bioactive complexes

Savić Ivan (Tehnološki fakultet, Leskovac)
Nikolić Goran (Tehnološki fakultet, Leskovac)
Savić Ivana (Tehnološki fakultet, Leskovac)
Cakić Milorad (Tehnološki fakultet, Leskovac)

The aim of this work was to optimize a GFC method for the analysis of bioactive metal (Cu, Co and Fe) complexes with olygosaccharides (dextran and pullulan). Bioactive metal complexes with olygosaccharides were synthesized by original procedure. GFC was used to study the molecular weight distribution, polymerization degree of oligosaccharides and bioactive metal complexes. The metal bounding in complexes depends on the ligand polymerization degree and the presence of OH groups in coordinative sphere of the central metal ion. The interaction between oligosaccharide and metal ions are very important in veterinary medicine, agriculture, pharmacy and medicine.

Keywords: bioactive complexes, molecular weight distribution, polymerization degree, gel-filtration chromatography