doiSerbia

In vitro examination of oral tissue conditioners potential toxicity

Krunić Nebojša (Clinic of Dentistry, Faculty of Medicine, Niš)
Nikolić Ljubiša (Faculty of Technology, Leskovac)
Kostić Milena (Department of Prosthodontics, Clinic of Dentistry, Faculty of Medicine, Niš)
Najman Stevo (Institute of Biomedical Researches, Faculty of Medicine, Niš)
Nikolić Vesna (Faculty of Technology, Leskovac)
Najdanović Jelena (Institute of Biomedical Researches, Faculty of Medicine, Niš)

The oral tissue conditioners are applied temporarily to the gingival surface of a denture for the purpose of reconditioning the abused denture supporting tissues, allowing them to return to a normal, healthy state. According to chemical composition they can be classified into methacrylic and silicon conditioners. The objective of this research was to examine the potential toxicity of one, seven and thirty day extracts of different concentrations. Different effective concentrations (5, 12.5, 25 and 50%) of two silicone and four methacrylic conditioners extracts were used after extraction period of one, seven and thirty days. Amount of toxic substances and dynamics of their release were monitored by high-pressure liquid chromatography (HPLC). HPLC analysis could not be applied to the silicone conditioners because they consist of polymeric and inorganic 19 substances. Evaluation of cell proliferation, as cytotoxic parameters, was done by Mosmann’s MTT assay based on reduction of yellow tetrazole (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - MTT) to purple formazan by mitochondrial succinate dehydrogenase of metabolic active cells. With increasing of extraction period, the amount of released potential toxic substances increased. The cytotoxicity of tested materials significantly increased with extract concentration increase and duration of extraction period. To improve the biological characteristics of mobile dentures relined by soft materials, soaking in water the day before insertion into patient’s mouth was recommended. Silicone conditioners showed less cytotoxicity compared to the methacrylic conditioners, so they are more appropriate for daily dental practice.

Keywords: cytotoxicity, oral tissue conditioners, MTT assay, HPLC