A method of mathematically analyzing interdendritic microsegregation was established using finite difference method and taking into consideration the diffusion of the solute in the solid and liquid phases. The cross-sectional shape of dendrites and the fact that the enrichment of the solute in the liquid phase at the solid-liquid interface restrains the advancement speed of the solid-liquid interface were considered. Directional solidification tests to examine interdendritic segregation were made to verify the mathematical analysis method established. The advantages of the new method over other methods were discussed. Then, spot-like segregations were mathematically analyzed applying the same method, and the results were in good agreement with the observations in continuously-cast slabs.