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Plant genetic transformation using carbon nanotubes for DNA delivery

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Abstract

The possibility of using nanocarriers based on carbon nanotubes (CNTs) to deliver genetic material into mesophyll protoplasts, callus cells, and leaf explants is discussed. Using single-walled CNTs (SWCNTs) at the concentration of 20 μg/mL and multiwalled CNTs (MWCNTs) at the concentration of 15 μg/mL, the Nicotiana tabacum L. protoplasts were genetically transformed with the plasmid construct pGreen 0029, and a transient expression of the yfp reporter gene was shown in the protoplasts. Using SWCNTs at the concentration of 40 μg/mL and MWCNTs at the concentration of 30 μg/mL, the N. tabacum callus and leaf explants were genetically transformed by the nptII gene contained in the pGreen 0029 construct and regenerant plants were obtained on a selective medium with kanamycin at the concentration of 50 mg/L. The SWCNTs-based nanocarriers demonstrated their applicability for the transformation of protoplasts and walled plant cells. At the same time, the MWCNTs-based nanocarriers demonstrated their applicability only for the transformation of protoplasts, because of a limiting role of the cellulose wall against their penetration into the cells.

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Authors and Affiliations

  1. Institute of Food Biotechnology and Genomics, Kyiv, Ukraine

    O. M. Burlaka, Ya. V. Pirko, A. I. Yemets & Ya. B. Blume

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  1. O. M. Burlaka
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  2. Ya. V. Pirko
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  3. A. I. Yemets
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  4. Ya. B. Blume
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Correspondence to O. M. Burlaka.

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Original Ukrainian Text © O.M. Burlaka, Ya.V. Pirko, A.I. Yemets, Ya.B. Blume, 2015, published in Tsitologiya i Genetika, 2015, Vol. 49, No. 6, pp. 3–12.

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Burlaka, O.M., Pirko, Y.V., Yemets, A.I. et al. Plant genetic transformation using carbon nanotubes for DNA delivery. Cytol. Genet. 49, 349–357 (2015). https://doi.org/10.3103/S009545271506002X

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  • DOI: https://doi.org/10.3103/S009545271506002X

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