Microwave-Assisted Extraction of Chlorophyll from Filter Mud of Sugercane Mill and Component Analysis

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Abstract:

Microwave-assisted extraction (MAE) was used to extract chlorophylls from filter mud. Ethanol was used as the solvent. The optimal conditions for the MAE of chlorophylls were concluded from the study as the irradiation time, 50 s, the ratio of liquid to solid, 8:1 (mL/g), the extraction temperature, 40 °C, and the extraction time, 60 min. Compared with conventional extraction, the MAE of chlorophylls from the filter mud was more effective. The extraction time for MAE was 60 min with 0.277 mg/g chlorophyll yield, while conventional extraction needed 240 min with only about 0.259 mg/g chlorophyll yield. The Ultraviolet Absorption Spectra of the extracted chlorophylls showed that there was a strong absorption peak at about 663 nm. C=N, Mg-N and C-N was not seen existed from the infrared spectroscopy probably because that the mixed extracts were not purified and the chlorophyll content was less.

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Periodical:

Advanced Materials Research (Volumes 518-523)

Pages:

430-435

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Online since:

May 2012

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[1] M.T. Elsayed, M.H. Babiker, M.E. Abdelmalik, O.N. Mukhtar, D. Montange. Bioresource Technology, 2008,99: 4164–4168.

DOI: 10.1016/j.biortech.2007.08.079

Google Scholar

[2] W.T. Tsai, H.P. Chen, C.W. Lai, K.J. Hsien, M.S. Lee, J.M. Yang. Journal of Analytical Applied Pyrolysis, 2003,70: 399–411.

Google Scholar

[3] Shiyi Ou, Jian Zhao, Yong Wang, Ye Tian, Jiong Wang. LWT - Food Science and Technology, 2012, 45:295–298.

Google Scholar

[4] F.Ben Rebah, D.Prévost, A.Yezza, R.D. Tyagi.Bioresource Technology,2007, 98:3535–3546.

Google Scholar

[5] E.T. Thostenson, T.-W. Chou. Composites: Part A, 1999,30:1055–1071.

Google Scholar

[6] Rhena Schumann, Norbert Ha¨ ubner, Steffi Klausch, Ulf Karsten. International Biodeterioration & Biodegradation, 2005, 55: 213–222.

DOI: 10.1016/j.ibiod.2004.12.002

Google Scholar

[7] M.D. Mac´ıas-S´anchez, C. Mantell, M. Rodr´ıguez, E. Mart´ınez de la Ossa, L.M. Lubi´an, O. Montero et al. J. of Supercritical Fluids, 2007,39:323–329.

Google Scholar

[8] A.R. Wellburn. J.Plant Physiol,1994,144:307–313.

Google Scholar

[9] Arnon DI(1949). Copper enzymes in isolated chloroplasts.Polyphenol oxidase in Beta vulgaris.Plant Physiol 24:1–5.

DOI: 10.1104/pp.24.1.1

Google Scholar

[10] Youn Yuen Shu, Ming Yu Ko, Yuan Shiun Chang. Microchemical Journal, 2003,74 :131–139.

Google Scholar

[11] Jin-yu Hao, Wei Han, Shun-de Huang, Bo-yong Xue, Xiu Deng. Separation and Purification Technology, 2002,28:191–196.

Google Scholar

[12] Catalina Cubas, M. Gloria Lobo, Mo´nica Gonza´lez. Journal of Food Composition and Analysis, 2008,21: 125–133.

Google Scholar

[13] G. Spigno, D.M. De Faveri. Journal of Food Engineering, 2009,93:210–217.

Google Scholar

[14] Youn Yuen Shu, Teh Long Lai, Huann-shyang Lin,Thomas C. Yang, Chi-Peng Chang. Chemosphere, 2003,52:1667–1676.

Google Scholar

[15] BUDZINSKI H, LETELLIER M, GARRIGUES P, et al. Journal of Chromatography A, 1999, 837(1/2): 187-200.

Google Scholar