In Vitro Biocompatibility Study of Nano TiO₂ Materials

Abstract:

Nano TiO2 material is an extensively used and adequately studied material and has a close contact with human in various fields, such as dope, dye, ceramic, cosmetic and medicine. Therefore, it’s very important to study the biocompatibility and biosafety of nano TiO2 materials. In the present study, various nano TiO2 materials with different dimension and crystal structures were confected to suspensions with varied concentrations and evaluated in cell model (mouse fibrocyte) after autoclaving sterilization. After 24h, 48h and 72h of cell culture experiments, MTT assay was used to examine the cell proliferation behavior and the flow cytometry was used to examine the cell apoptosis behavior. The present results of cell experiment showed that nano TiO2 materials had no effect on cell proliferation and apoptosis in a certain range of time and concentration. MTT assay indicated the relative cell proliferation rate in all nano TiO2 material groups were above 92% and the toxicity grade were 0 or 1 class.