Fluorescence Silica Nanoprobe as a Biomarker for Rapid Detection of Plant Pathogens

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Abstract:

Fluorescent silica nanoprobe as a biomarker for detection has attracted much attention in the field of nano-biotechnology recently but no further research applications using fluorescent silica nanoparticles (FSNP) combined with antibody molecules reported to detect pathogen detection. In this study, silica nanoparticles were prepared using the water-in-oil (w/o) microemulsion method. The silica nanoparticles were circular in diameter of 50 ± 4.2 nm. The organic dye, tris-2, 2' -bipyridyl dichlororuthenium (II) hexahydrate (Rubpy), could be incorporated efficiently into the core of silica nanoparticles. The fluorescence of Rubpy-doped silica nanoparticles was photostable using a collisional quenching fluorescence test. The Rubpy-doped silica nanoparticles were conjugated with the secondary antibody of goat anti-rabbit immunoglobulin G (IgG) and successfully detected plant pathogen such as Xanthomonas axonopodis pv. vesicatoria that causes bacterial spot disease in Solanaceae plant. These results demonstrated that the fluorescence silica nanoprobe biomarker will have been potential for rapid diagnosis applications on plant diseases.

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Periodical:

Advanced Materials Research (Volumes 79-82)

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513-516

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Online since:

August 2009

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[1] O. Grankvist, L. Walther, U. Bredberg-Raden, E. Lyamuya, F. Mhalu, A. Gustafsson, A. G. Biberfeld and G., Wadell. J. Virol. Meth. Vol. 62 (1996), p.131.

DOI: 10.1016/s0166-0934(96)02094-0

Google Scholar

[2] W. E. Moerner and M. Orit. Science Vol. 283 (1999), p.1670.

Google Scholar

[3] M. J. Bruchez, M. Moronne, P. Gin, S. Weiss and A. P. Alivisatos. Science Vol. 281 (1998), p. (2013).

Google Scholar

[4] J. Adler, A. Jayan and C. D. Melia. J. Pharm. Sci. Vol. 88 (1999), p.371.

Google Scholar

[5] S. Santra, P. Zhang, K. M. Wang, R. Tapec and W. Tan. Anal. Chem. Vol. 73 (2001), p.4988.

Google Scholar

[6] S. Santra, K. M. Wang, R. Tapec and W. Tan. J. Biomed. Opt. Vol. 6 (2001), p.160.

Google Scholar

[7] W. H. Liu, C. Hong, C. S. Chen and M.C. Yeh. J. Chin. Electron Microsc. Soc. Vol. 23 (2004), p.225.

Google Scholar

[8] H. Bouzar, J.B. Jones, R.E. Stall, N.C. Hodge, G.V. Minsavage, A.A. Benedict and A.M. Alvarez. Phytopathology Vol. 84 (1994), p.663.

Google Scholar

[9] R.P. Leite, J.B. Jones and G.C. Somodi. Plant Dis. Vol. 79 (1995), p.917.

Google Scholar

[10] S. Mitrev and B. Kovačevič. J. Plant Pathol. Vol. 88 (2006), p.321.

Google Scholar

[11] Information on http: /www. eppo. org/QUARANTINE/quarantine. htm.

Google Scholar

[12] W. Yang, C. G. Zhang, H. Y. Qu, H. H Yang and J. G. Xu, Anal. Chim. Acta Vol. 503 (2004), p.163.

Google Scholar

[13] W. Lian , A. S. Litherland , H. Badrane , W. Tan , D. Wu ,H. V. Baker , P. A. Gulig , D. V. Lim and S. Jin, Anal. Biochem. Vol. 334 (2004), p.135.

DOI: 10.1016/j.ab.2004.08.005

Google Scholar

[14] G. Lambert, E. Fattal, H. Pinto-Alphandary, A. Gulik and P. Couvreur, Pharm. Res. Vol. 17(2000), p.707.

DOI: 10.1023/a:1007582332491

Google Scholar

[15] W. Yang, C. G. Zhang, H. Y. Qu, H. H. Yang and J. G. Xu, Anal. Chim. Acta Vol. 503 (2004), p.163.

Google Scholar

[16] X. Zhao, L. R. Hilliard, S. J. Mechery, Y. Wang, R. P. Bagwwe and S. Jin, Proc. Natl. Acad. Sci. U.S.A. Vol. 101 (2004), p.15027.

Google Scholar

[17] R.J. McLaughlin and T.A. Chen, in: Serological methods for detection and identification of viral and bacterial plant pathogens. A laboratory manual, edited by R. Hampton, E. Ball, and S. De Boer. Pages 197-204. APS Press (1990).

Google Scholar

[18] K.S. Yao, S.J. Li and K.C. Tzeng, Plant Pathol. Bull. Vol. 17 (2008), p.81.

Google Scholar

[19] J. Andrey and J. Y. Ying, Nature Vol. 403 (2000), p.65.

Google Scholar

[20] J. Kiler, C. J. Tucker, T. H. Kalantra and D. P. Green, Adv. Mater. Vol. 23 (2000), p.1751.

Google Scholar

[21] S. Santra, R. Tapec, N. Theodoropoulou and J. Dobson, Langmuir Vol. 10 (2001), p.2900.

Google Scholar

[22] A. V. Blaaderen and A. Vrij, Langmuir Vol. 8 (1992), p.2921.

Google Scholar

[23] S. Phadtare, A. Kumar, V. P. Vinod, C. Dash, D.V. Palaskar, M. Rao, P. G. Shukla, S. Sivaram and M. Sastry, Chem. Mater. Vol. 15 (2003), p. (1944).

DOI: 10.1021/cm020784a

Google Scholar

[24] Z. Lin, S. Cui, H. Zhang, Q. Chen, B. Yang, X. Su, J. Zhang and Q. Jin, Anal. Biochem. Vol. 319 (2003), p.239.

Google Scholar