Abstract

In this study, the feasibility of using rice grass (Spartina spp.) as the main substrate for cellulase production by a newly isolated strain Aspergillus sp. SEMCC-3.248 under solid-state fermentation was evaluated. The optimized medium and culture conditions were: rice grass (0.8-0.9 mm) 2.5 g, Wheat bran 1.5 g, 4 ml of nutrient solution ((NH₄)₂SO₄ 14 g/L, KH₂PO₄ 2 g/L, CaCl₂ 4 g/L, MgSO₄·7H₂O 0.2 g/L, soluble starch 2.62 g/L, peptone 1.51 g/L), 1 ml of spore suspension (1×10⁷ spores/ml), initial moisture content 70%, initial pH 5.0, incubation temperature 32°C and fermentation period 5 days. Under these conditions, the cellulase activity could reach 1.14 FPIU/gds.

Key words: Cellulase, isolation, identification, solid-state fermentation, Spartina spp.,Aspergillus sp.