Chemical Synthesis of Silver Nanoparticles Using Tri-sodium Citrate, Stability Study and Their Characterization
Prem Santhi Yerragopu *
Department of Processing and Food Engineering, College of Agricultural Engineering, University of Agricultural Sciences, Raichur, India.
Sharanagouda Hiregoudar
Department of Processing and Food Engineering, College of Agricultural Engineering, University of Agricultural Sciences, Raichur, India.
Udaykumar Nidoni
Department of Processing and Food Engineering, College of Agricultural Engineering, University of Agricultural Sciences, Raichur, India.
K. T. Ramappa
Department of Processing and Food Engineering, College of Agricultural Engineering, University of Agricultural Sciences, Raichur, India.
A. G. Sreenivas
Department of Agricultural Entomology, College of Agriculture, University of Agricultural Sciences, Raichur, India.
S. R. Doddagoudar
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Raichur - 584 101, Karnataka, India.
*Author to whom correspondence should be addressed.
Abstract
The present work was aimed to study the synthesis of silver nanoparticles (Ag NPs) using Tri-Sodium Citrate (TSC), stability study of synthesized Ag NPs and their characterization. Synthesis of Ag NPs has been carried out by maintaining different conditions such as TSC concentration (0.50, 1.00 and 1.50%), AgNO3 concentration (0.50, 1.00 and 1.50 mM) and stirring time (10, 15 and 20 min). The stability study of synthesized Ag NPs conducted for 30 days without adding any stabilizing agents. The characterization of synthesized Ag NPs was carried out for different parameters like particle size and zeta potential using particle size analyzer, absorbance peak by UV-Visible spectrophotometer, morphology by Scanning Electron Microscope (SEM), crystallinity by X-Ray Diffractometer (XRD) and material structural characteristics by Atomic Force Microscope (AFM). The stable chemically synthesized Ag NPs were obtained at C20 (AgNO3 concentration of 1.5 mM, TSC 1.5% and stirring time 20 min) (desirability 99.97%), with average particle size of 22.14 nm and average absorbance peak of 449.85 nm.
Keywords: Absorbance peak, characterization, tri-sodium citrate, particle size, silver nanoparticles and stability study