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Erschienen in: Cellulose 2/2013

01.04.2013 | Original Paper

Mechanical deconstruction of lignocellulose cell walls and their enzymatic saccharification

verfasst von: Ingrid C. Hoeger, Sandeep S. Nair, Arthur J. Ragauskas, Yulin Deng, Orlando J. Rojas, J. Y. Zhu

Erschienen in: Cellulose | Ausgabe 2/2013

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Abstract

Laboratory mechanical softwood pulps (MSP) and commercial bleached softwood kraft pulps (BSKP) were mechanically fibrillated by stone grinding with a SuperMassColloider®. The extent of fibrillation was evaluated by SEM imaging, water retention value (WRV) and cellulase adsorption. Both lignin content and mechanical treatment significantly affected deconstruction and enzymatic saccharification of fibrillated MSP and BSKP. Fibrillation of MSP and BSKP cell walls occurs rapidly and then levels off; further fibrillation has only limited effect on cell wall breakdown as measured by water retention value and cellulase adsorption. Complete (100 %) saccharification can be achieved at cellulase loading of 5 FPU/g glucan for BSKP after only 15 min fibrillation with energy input of 0.69 MJ/kg. However, the presence of lignin in MSP affects the extent of fibrillation producing fibrils mainly above 1 μm. Lignin binds nonproductively to cellulases and blocks cellulose thereby reducing its accessibility. As a result, the cellulose saccharification efficiency of MSP fibrils (6 h of fibrillation, energy input of 13.33 MJ/kg) was only 55 % at same cellulase loading of 5 FPU/g glucan.

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Metadaten
Titel
Mechanical deconstruction of lignocellulose cell walls and their enzymatic saccharification
verfasst von
Ingrid C. Hoeger
Sandeep S. Nair
Arthur J. Ragauskas
Yulin Deng
Orlando J. Rojas
J. Y. Zhu
Publikationsdatum
01.04.2013
Verlag
Springer Netherlands
Erschienen in
Cellulose / Ausgabe 2/2013
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-013-9867-9

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