nach oben

Journal of Nanoparticle Research

Erschienen in:

01.10.2012 | Research Paper

Methotrexate-conjugated magnetic nanoparticles for thermochemotherapy and magnetic resonance imaging of tumor

verfasst von: Fuping Gao, Zixing Yan, Jing Zhou, Yuanyuan Cai, Jintian Tang

Erschienen in: Journal of Nanoparticle Research | Ausgabe 10/2012

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config

KI-gestützte Suche

Aus

Abstract

There is significant interest in recent years in developing magnetic nanoparticles (MNPs) having multifunctional characteristics with complimentary roles. In this study, methotrexate (MTX) was conjugated on the iron oxide magnetic nanoparticles surface via a poly(ethyleneimine) self-assembled monolayer (MTX–MNPs). The novel platform combined cancer chemotherapy, hyperthermia and potential monitoring of the progression of disease through magnetic resonance imaging (MRI). The conjugation of MTX on the magnetite surface was confirmed by Fourier transform infrared spectroscopy and change of zeta potential. Transmission electron microscope (TEM) showed that MTX–MNPs were morphologically spherical. The average diameter of MTX–MNPs was 30.1 ± 5.2 nm determined by dynamic light scattering. Magnetic measurements revealed that the saturation magnetization of MTX–MNPs reached 68.8 emu/g and the nanoparticles were superparamagnetic. The MTX–MNPs had good heating properties in an alternating magnetic field. TEM results showed that a larger number of MTX–MNPs were internalized into the MCF-7 cellular cytoplasm compared with the MNPs. The MTX–MNPs demonstrated highly synergistic antiproliferative effects of simultaneous chemotherapy and hyperthermia in MCF-7 breast cancer cells. A significant negative contrast enhancement was observed with magnetic resonance phantom imaging for MCF-7 cells over L929cells, when both were cultured with the nanoconjugate. The MTX–MNPs with combined characteristics of thermochemotherapy and MRI could be of high clinical significance in the treatment of tumor.

Vorheriger Artikel Magnetic field assisted polyol synthesis of cobalt carbide and cobalt microwires

Nächster Artikel Influence of the synthetic polypeptide c25-mms6 on cobalt ferrite nanoparticle formation

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Arruebo M, Fernández-Pacheco R, Ibarra MR, Santamaría J (2007) Magnetic nanoparticles for drug delivery. Nano Today 2:22–32CrossRef

Banerjee D, Mayer-Kuckuk P, Capiaux G, Budak-Alpdogan T, Gorlick R, Bertino JR (2002) Novel aspects of resistance to drugs targeted to dihydrofolate reductase and thymidylate synthase. Biochim Biophys Acta 1587:164–173CrossRef

Brusentsov NA, Brusentsova TN, Filinova EY, Jurchenko NY, Kupriyanov DA, Pirogov YA, Dubina AI, Shumskikhd MN, Shumakov LI, Anashkina EN et al (2007) Magnetohydrodynamic thermochemotherapy and MRI of mouse tumors. J Magn Magn Mater 311:176–180CrossRef

Bulte JWM, Kraitchman DL (2004) Iron oxide MR contrast agents for molecular and cellular imaging. NMR Biomed 17:484–499CrossRef

Corot C, Rober P, Idée J-M, Port M (2006) Recent advances in iron oxide nanocrystal technology for medical imaging. Adv Drug Deliv Rev 58:1471–1504CrossRef

Cronstein BN, Bertino JR (2000) Methotrexate. Birkhauser Verlag Basel, Switzerland, pp 9–49CrossRef

Dube D, Francis M, Leroux JC, Winnik FM (2002) Preparation and tumor cell uptake of poly(N-isopropylacrylamide) folate conjugates. Bioconjug Chem 13:685–692CrossRef

Duthie SJ (2001) Folic-acid-mediated inhibition of human colon-cancer cell growth. Nutrition 17:736–737CrossRef

Gao J, Gu H, Xu B (2009) Multifunctional magnetic nanoparticles: design, synthesis, and biomedical applications. Acc Chem Res 42:1097–1107CrossRef

Gao F, Cai Y, Zhou J, Xie X, Ouyang W, Zhang Y, Wang X, Zhang X, Wang X, Tang J et al (2010) Pullulan acetate coated magnetite nanoparticles for hyperthermia: preparation, characterization and in vitro experiments. Nano Res 3:23–31CrossRef

Gao F, Li L, Liu T, Hao N, Liu H, Tan L, Li H, Huang X, Peng B, Tang FQ et al (2012) Doxorubicin loaded silica nanorattle actively homing to tumor with improved anti-tumor effect. Nanoscale 4:3365–3372CrossRef

Harisinghani MG, Barentsz J, Hahn PF, Deserno WM, Tabatabaei S, van de Kaa CH, de la Rosette J, Weissleder R (2003) Noninvasive detection of clinically occult lymph-node metastases in prostate cancer. N Engl J Med 348:2491–2499CrossRef

Huh YM, Jun YW, Song HT, Kim S, Choi JS, Lee JH, Yoon S, Kim KS, Shin JS, Cheon J et al (2005) In vivo magnetic resonance detection of cancer by using multifunctional magnetic nanocrystals. J Am Chem Soc 127:12387–12391CrossRef

Islam T, Harisinghani MG (2009) Overview of nanoparticle use in cancer imaging. Cancer Biomark 5:61–67

Ito A, Fujioka M, Yoshida T, Wakamatsu K, Ito S, Yamashita T, Jimbow K, Honda H (2007) 4-S-Cysteaminephenol-loaded magnetite cationic liposomes for combination therapy of hyperthermia with chemotherapy against malignant melanoma. Cancer Sci 98:424–430CrossRef

Jain TK, Richey J, Strand M, Leslie-Pelecky DL, Flask CA, Labhasetwar V (2008) Magnetic nanoparticles with dual functional properties: drug delivery and magnetic resonance imaging. Biomaterials 29:4012–4021CrossRef

Jing Y, Moore LR, Williams PS, Chalmers JJ, Farag SS, Bolwell B, Zborowski M (2007) Blood progenitor cell separation from clinical leukapheresis product by magnetic nanoparticle binding and magnetophoresis. Biotechnol Bioeng 96:1139–1154CrossRef

Johannsen M, Thiesen B, Wust P, Jordan A (2010) Magnetic nanoparticle hyperthermia for prostate cancer. Int J Hyperth 26:790–795CrossRef

Kamruzzaman Selim KM, Ha YS, Kim SJ, Chang Y, Kim TJ, Lee GH, Kang IK (2007) Surface modification of magnetite nanoparticles using lactobionic acid and their interaction with hepatocytes. Biomaterials 28:710–716CrossRef

Kohler N, Sun C, Fichtenholtz A, Gunn J, Fang C, Zhang M (2006) Methotrexate-immobilized poly(ethylene glycol) magnetic nanoparticles for MR imaging and drug delivery. Small 2:785–792CrossRef

Li GY, Huang KL, Jiang YR, Ding P, Yang DL (2008) Preparation and characterization of carboxyl functionalization of chitosan derivative magnetic nanoparticles. Biochem Eng J 40:408–414CrossRef

McBain SC, Yiu HHP, Dobson J (2008) Magnetic nanoparticles for gene and drug delivery. Int J Nanomed 3:169–180

Medarova Z, Pham W, Kim Y, Dai GP, Moore A (2006) In vivo imaging of tumor response to therapy using a dual-modality imaging strategy. Int J Cancer 118:2796–2802CrossRef

Mikhaylova M, Kim DK, Berry CC, Zagorodni A, Toprak M, Curtis ASG, Muhammed M (2004) BSA immobilization on amine-functionalized superparamagnetic iron oxide nanoparticles. Chem Mater 16:2344–2354CrossRef

Mohamed F, Marchettini P, Stuart OA, Urano M, Sugarbaker PH (2003) Thermal enhancement of new chemotherapeutic agents at moderate hyperthermia. Ann Surg Oncol 10:463–468CrossRef

Morishige K, Kacher DF, Libby P, Josephson L, Ganz P, Weissleder R, Aikawa M (2010) High-resolution magnetic resonance imaging enhanced with superparamagnetic nanoparticles measures macrophage burden in atherosclerosis. Circulation 122:1707–1715CrossRef

Park JY, Daksha P, Lee GH, Woo S, Chang Y (2008) Highly water-dispersible PEG surface modified ultra small superparamagnetic iron oxide nanoparticles useful for target-specific biomedical applications. Nanotechnology 19:365603–365609CrossRef

Pradhan P, Giri J, Rieken F, Koch C, Mykhaylyk O, Döblinger M, Banerjee R, Bahadur D, Plank C (2010) Targeted temperature sensitive magnetic liposomes for thermo-chemotherapy. J Control Release 142:108–121CrossRef

Purushotham S, Ramanujan RV (2010) Thermoresponsive magnetic composite nanomaterials for multimodal cancer therapy. Acta Biomater 6:502–510CrossRef

Riehemann K, Schmitt O, Ehlers EM (2005) The effects of thermochemotherapy using cyclophosphamide plus hyperthermia on the malignant pleural mesothelioma in vivo. Ann Anat 187:215–223CrossRef

Sanson C, Diou O, Thevenot J, Ibarboure E, Soum A, Brulet A, Miraux S, Thiaudiere E, Tan S, Brisson A, Dupuis V, Sandre O, Lecommandoux S (2011) Doxorubicin loaded magnetic polymersomes: theranostic nanocarriers for MR imaging and magneto-chemotherapy. ACS Nano 5:1122–1140CrossRef

Seo SB, Yang J, Hyung W, Cho EJ, Lee TI, Song YJ, Yoon HG, Suh JS, Huh YM, Haam S (2007) Novel multifunctional PHDCA/PEI nano-drug carriers for simultaneous magnetically targeted cancer therapy and diagnosis via magnetic resonance imaging. Nanotechnology 18:475105CrossRef

Sudimack J, Lee RJ (2000) Targeted drug delivery via the folate receptor. Adv Drug Deliv Rev 41:147–162CrossRef

Sun C, Sze R, Zhang M (2006) Folic acid-PEG conjugated superparamagnetic nanoparticles for targeted cellular uptake and detection by MRI. J Biomed Mater Res A 78:550–557

Takemoto M, Kuroda M, Urano M, Nishimura Y, Kawasaki S, Kato H, Okumura Y, Akaki S, Kanazawa S, Hiraki Y et al (2003) The effect of various chemotherapeutic agents given with mild hyperthermia on different types of tumours. Int J Hyperth 19:193–203CrossRef

Thiesen B, Jordan A (2008) Clinical applications of magnetic nanoparticles for hyperthermia. Int J Hyperth 24:467–474CrossRef

Trippett TM, Garcia S, Manova K, Mody R, Cohen-Gould FlintoffW, Bertino JR (2001) Localization of a human reduced folate carrier protein in the mitochondrial as well as the cell membrane of leukemia cells. Cancer Res 61:1941–1947

Wang X, Zhou L, Ma Y, Li X, Gu H (2009) Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection. Nano Res 2:365–372CrossRef

Wang L, Neoh KG, Kang ET, Shuter B, Wang SC (2010) Biodegradable magnetic-fluorescent magnetite/poly(dl-lactic acid-co-α, β-malic acid) composite nanoparticles for stem cell labeling. Biomaterials 31:3502–3511CrossRef

Weissleder R, Kelly K, Sun EY, Shtatland T, Josephson L (2005) Cell specific targeting of nanoparticles by multivalent attachment of small molecules. Nat Biotechnol 23:1418–1423CrossRef

Yallapu MM, Othman SF, Curtis ET, Gupta BK, Jaggi M, Chauhan SC (2011) Multi-functional magnetic nanoparticles for magnetic resonance imaging and cancer therapy. Biomaterials 32:1890–1905CrossRef

Yan G-P, Robinson L, Hogg P (2007) Magnetic resonance imaging contrast agents: overview and perspectives. Radiography 13:e5–e19CrossRef

Titel: Methotrexate-conjugated magnetic nanoparticles for thermochemotherapy and magnetic resonance imaging of tumor
verfasst von: Fuping Gao
Zixing Yan
Jing Zhou
Yuanyuan Cai
Jintian Tang
Publikationsdatum: 01.10.2012
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 10/2012
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-012-1160-6

Premium Partner

Marktübersichten

Die im Laufe eines Jahres in der „adhäsion“ veröffentlichten Marktübersichten helfen Anwendern verschiedenster Branchen, sich einen gezielten Überblick über Lieferantenangebote zu verschaffen.

Zur Marktübersicht

Springer Professional

Abstract

Bitte loggen Sie sich ein, um Zugang zu Ihrer Lizenz zu erhalten.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Weitere Artikel der Ausgabe 10/2012

Magnetic field assisted polyol synthesis of cobalt carbide and cobalt microwires

Novel methods for the synthesis of magnetite nanoparticles with special morphologies and textured assemblages

Relative binding affinity of carboxylate-, phosphonate-, and bisphosphonate-functionalized gold nanoparticles targeted to damaged bone tissue

Spectroscopic and microscopic investigation of gold nanoparticle nucleation and growth mechanisms using gelatin as a stabilizer

Doughnut magnesium fluoride nanoparticles prepared by an electron-beam irradiation method

Fluorescence quantum efficiency of CdSe/ZnS quantum dots functionalized with amine or carboxyl groups

Premium Partner

Marktübersichten