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Erschienen in:
2014 | OriginalPaper | Buchkapitel
Microbioreactor Integrated with a Sensor for Monitoring Intracellular Green Fluorescence Protein (GFP)
verfasst von : Godfrey Pasirayi, Meez Islam, Simon M. Scott, Liam O’Hare, Zulfiqur Ali
Erschienen in: XIII Mediterranean Conference on Medical and Biological Engineering and Computing 2013
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A prototype Polydimethylsiloxane (PDMS) based microbioreactor integrated with a photodiode detector for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression is described. The developed system is compact, simple and inexpensive thus making it ideal for an economical on chip detection of intracellular GFP and other biological applications. The detection limit for cell free GFPUV was found to be 4.8 ×10
− 8
M while for Fluorescein isothiocyanate (FITC) was found to be 1.2 ×10
− 6
M. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. This study demonstrates the quest to develop integrated microsystems that can be used to monitor cellular dynamics in real time.