Microreactions on the Surface of an Oligonucleotide Bearing Chip

Plain surfaces with suitable chemical functions at defined positions are prepared. The handling of such kind of microreactors is simplified enormously since many probes defined by their chemical nature and their position are treated simultaneously. However the reaction products are different depending on the immobilized probe. Enzymatic reactions are becoming a valuable tool in order to achieve high specifity and efficiency. Typical reactions in molecular biology such as hybridization and nuclease digestion have been carried out on the surface of on oligonucleotide bearing chip. The density of molecules immobilized onto the surface influences the efficiency of the desired reaction. Gelatine as the main component of the immobilization matrix proofed to be a suitable material to set up a micropatterned multi functional immobilization matrix.