2015 | OriginalPaper | Buchkapitel
Multiparametric Biosensor for Detection and Monitoring of Bacterial Biofilm Adhesion and Growth
verfasst von : Sheila Becerro, Jacobo Paredes, Sergio Arana
Erschienen in: 6th European Conference of the International Federation for Medical and Biological Engineering
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Bacterial biofilms cause numerous problems in a wide variety of sectors as the medical environment, the food and water industry or the naval sector. The detection of the infection is usually made in advanced states when treatment is hampered by the antibiotic resistance of biofilms. During the first phases, there are not evidential indicators that warn about the presence of pathogens. Therefore, it is necessary to find new methods for the early detection of biofilm development so as to improve the efficiency of treatments. For this purpose, this paper focuses on the design and development of interdigitated microelectrode based biosensors that allow the detection of bacterial adhesion since the first steps of biofilm generation through electrochemistry and two supplementary physicochemical parameters: temperature and pH. The designed biosensor has been proved as a suitable tool for biofilm detection since the first steps of development by means of differential pulse voltammetry and temperature measurements.