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Erschienen in: Journal of Nanoparticle Research 1/2016

01.01.2016 | Research Paper

Oxidative stress-mediated cytotoxicity of zirconia nanoparticles on PC12 and N2a cells

verfasst von: Elham Asadpour, Hamid R. Sadeghnia, Ahmad Ghorbani, Mehran Sedaghat, Mohammad T. Boroushaki

Erschienen in: Journal of Nanoparticle Research | Ausgabe 1/2016

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Abstract

In recent years, there is a growing interest in the application of nanoparticles like zirconium dioxide (zirconia <100 nm), for many purposes. Since a comprehensive study on the toxic effects of zirconia has not been done, we decided to investigate the effects of zirconia nanoparticles on cultured PC12 and N2a cells. In this study, cytotoxic effect of different concentrations of zirconia nanoparticles at three different time intervals were evaluated using MTT and ROS (reactive oxygen species) assays. Also, Lipid peroxidation, glutathione (GSH) content changes, and DNA damage were measured. Zirconia nanoparticles caused a significant reduction in cell viability and GSH content of the cells, and induce a significant increase in intracellular ROS and MDA content of PC12 and N2a cells. Moreover, it increases the percentage of DNA tail of treated cells as compared with control group. Zirconia nanoparticles have cytotoxic and genotoxic effects in PC12 and N2a cells in a time and concentration-dependent manner in concentration more than 31 µg/mL.

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Metadaten
Titel
Oxidative stress-mediated cytotoxicity of zirconia nanoparticles on PC12 and N2a cells
verfasst von
Elham Asadpour
Hamid R. Sadeghnia
Ahmad Ghorbani
Mehran Sedaghat
Mohammad T. Boroushaki
Publikationsdatum
01.01.2016
Verlag
Springer Netherlands
Erschienen in
Journal of Nanoparticle Research / Ausgabe 1/2016
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-015-3316-7

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