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Erschienen in: Cellulose 9/2020

09.04.2020 | Original Research

Preparation and characterization of bamboo fiber/chitosan/nano-hydroxyapatite composite membrane by ionic crosslinking

verfasst von: Shuo Tang, Liuyun Jiang, Bingli Ma, Chunyan Tang, Yue Wen, Na Zhang, Yan Zhang, Shengpei Su

Erschienen in: Cellulose | Ausgabe 9/2020

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Abstract

To obtain an ideal guided bone tissue regeneration (GBR) membrane, carboxylated bamboo fiber (BF) was designed to be introduced into the chitosan/nano-hydroxyapatite (CS/n-HA) system by ionic crosslinking to fabricate a novel BF/CS/n-HA composite membranes with different weight ratios. The formation mechanism of the BF/CS/n-HA ternary composite membrane and the effect of BF different contents on the BF/CS/n-HA composite membranes were studied by Fourier Transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), electromechanical universal tester, in vitro soaking in simulated body fluid (SBF). Results demonstrated that CS and modified BF would be ionic crosslinked by electrostatic interaction to form a three dimensional polyelectrolyte structure, meanwhile, n-HA was loaded in the polyelectrolyte structure by hydrogen bonding, which ensured the modified BF/CS/n-HA composite membranes to possess excellent tensile strength, compared to the CS/n-HA and untreated BF/CS/n-HA composite membrane. Moreover, the weight ratio of the modified BF/CS/n-HA of 2:6:2 displayed the highest tensile strength, whose tensile strength was improved by over two folds than that of CS/n-HA composite membrane owing to the most suitable proportion. In addition, in vitro simulated body fluids soaking results indicated that the modified BF/CS/n-HA composite membranes presented different degradation rates and could promote the apatite to deposit by investigating the weight loss, water absorption rate, SEM morphology observation and tensile strength reduce. All results revealed that the ionic crosslinking of carboxylated BF and CS was conducive to fabricate BF/CS/n-HA composite membrane with higher mechanical property, more suitable degradation and better bioactivity, in contrast with the CS/n-HA and untreated BF/CS/n-HA composite membranes, which had a great promising to obtain more satisfactory GBR membrane, moreover, the study would provide a new application for natural BF in the biomedical membrane field.

Graphic abstract

In this manuscript, carboxylated bamboo fiber (BF) was designed to be introduced into the chitosan/nano-hydroxyapatite (CS/n-HA) system to fabricate a novel BF/CS/n-HA composite membranes with different weight ratios. The formation mechanism of the BF/CS/n-HA ternary composite membrane and the effect of modified BF different contents on the BF/CS/n-HA composite membranes were studied, and the main purpose of the study was to explore the feasibility of preparing the new BF/CS/n-HA composite membrane by ionic crosslinking. Results revealed that the ionic crosslinking of carboxylated BF and CS played an essential role in fabricating BF/CS/n-HA composite membrane with higher mechanical properties, more suitable degradation and better bioactivity. The study proved that the carboxylated BF/CS/n-HA composite membrane fabricated by ionic crosslinking had a great potential in obtaining more satisfactory GBR membrane in contrast with the CS/n-HA and untreated BF/CS/n-HA composite membranes, which would open up a new application for natural BF in the biomedical membrane field.
Literatur
Zurück zum Zitat Aryaei A, Liu J, Jayatissa AH, Jayasuriya AC (2015) Cross-linked chitosan improves the mechanical properties of calcium phosphate-chitosan cement. Mat Sci Eng C-Mater 54:14–19 Aryaei A, Liu J, Jayatissa AH, Jayasuriya AC (2015) Cross-linked chitosan improves the mechanical properties of calcium phosphate-chitosan cement. Mat Sci Eng C-Mater 54:14–19
Zurück zum Zitat Baheti V, Militky J, Marsalkova M (2013) Mechanical properties of poly lactic acid composite films reinforced with wet milled jute nanofibers. Polym Compos 34:2133–2141 Baheti V, Militky J, Marsalkova M (2013) Mechanical properties of poly lactic acid composite films reinforced with wet milled jute nanofibers. Polym Compos 34:2133–2141
Zurück zum Zitat Bierhalz ACK, Moraes AM (2017) Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor. Mat Sci Eng C-Mater 76:287–294 Bierhalz ACK, Moraes AM (2017) Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor. Mat Sci Eng C-Mater 76:287–294
Zurück zum Zitat Bottino MC, Thomas V, Schmidt G, Vohra YK, Chu TMG, Kowolik MJ, Janowski GM (2012) Recent advances in the development of GTR/GBR membranes for periodontal regeneration-A materials perspective. Dent Mater 8:703–721 Bottino MC, Thomas V, Schmidt G, Vohra YK, Chu TMG, Kowolik MJ, Janowski GM (2012) Recent advances in the development of GTR/GBR membranes for periodontal regeneration-A materials perspective. Dent Mater 8:703–721
Zurück zum Zitat Cao LH, Weng WZ, Chen X, Ding YT, Yan YG, Li HH, Zhao H, Shin JW, Wei J, Ji F, Su JC (2015) Development of degradable and bioactive composite as bone implants by incorporation of mesoporous bioglass into poly(l-lactide). Composites B 77:454–461 Cao LH, Weng WZ, Chen X, Ding YT, Yan YG, Li HH, Zhao H, Shin JW, Wei J, Ji F, Su JC (2015) Development of degradable and bioactive composite as bone implants by incorporation of mesoporous bioglass into poly(l-lactide). Composites B 77:454–461
Zurück zum Zitat Dias LL, Mansur HS, Donnici CL, Pereira MM (2011) Synthesis and characterization of chitosan-polyvinyl alcohol-bioactive glass hybrid membranes. Biomatter 1:114–119 PubMedPubMedCentral Dias LL, Mansur HS, Donnici CL, Pereira MM (2011) Synthesis and characterization of chitosan-polyvinyl alcohol-bioactive glass hybrid membranes. Biomatter 1:114–119 PubMedPubMedCentral
Zurück zum Zitat Elgali I, Turri A, Xia W, Norlindh B, Johansson A, Dahlin C, Thomsen P, Omar O (2016) Guided bone regeneration using resorbable membrane and different bone substitutes: early histological and molecular events. Acta Biomater 29:409–423 Elgali I, Turri A, Xia W, Norlindh B, Johansson A, Dahlin C, Thomsen P, Omar O (2016) Guided bone regeneration using resorbable membrane and different bone substitutes: early histological and molecular events. Acta Biomater 29:409–423
Zurück zum Zitat Ezati M, Safavipour H, Houshmand B, Faghihi S (2018) Development of a PCL/gelatin/chitosan/beta-TCP electrospun composite for guided bone regeneration. Prog Biomater 7:225–237 PubMedPubMedCentral Ezati M, Safavipour H, Houshmand B, Faghihi S (2018) Development of a PCL/gelatin/chitosan/beta-TCP electrospun composite for guided bone regeneration. Prog Biomater 7:225–237 PubMedPubMedCentral
Zurück zum Zitat Grigoriadou I, Nianias N, Hoppe A, Terzopoulou Z, Bikiaris D, Will J, Hum J, Roether JA, Detsch R, Boccaccini AR (2014) Evaluation of silica-nanotubes and strontium hydroxyapatite nanorods as appropriate nanoadditives for poly(butylene succinate) biodegradable polyester for biomedical applications. Composites B 60:49–59 Grigoriadou I, Nianias N, Hoppe A, Terzopoulou Z, Bikiaris D, Will J, Hum J, Roether JA, Detsch R, Boccaccini AR (2014) Evaluation of silica-nanotubes and strontium hydroxyapatite nanorods as appropriate nanoadditives for poly(butylene succinate) biodegradable polyester for biomedical applications. Composites B 60:49–59
Zurück zum Zitat Guan QQ, Chen JJ, Chen D, Chai XS, He L, Peng LC, Zhang JH, Li J (2019) A new sight on the catalytic oxidation kinetic behaviors of bamboo cellulose fibers under TEMPO-oxidized system: the fate of carboxyl groups in treated pulps. J Catal 370:304–309 Guan QQ, Chen JJ, Chen D, Chai XS, He L, Peng LC, Zhang JH, Li J (2019) A new sight on the catalytic oxidation kinetic behaviors of bamboo cellulose fibers under TEMPO-oxidized system: the fate of carboxyl groups in treated pulps. J Catal 370:304–309
Zurück zum Zitat Guimaraes M, Botaro VR, Novack KM, Teixeira FG, Tonoli GHD (2015) Starch/PVA-based nanocomposites reinforced with bamboo nanofibrils. Ind Crops Prod 70:72–83 Guimaraes M, Botaro VR, Novack KM, Teixeira FG, Tonoli GHD (2015) Starch/PVA-based nanocomposites reinforced with bamboo nanofibrils. Ind Crops Prod 70:72–83
Zurück zum Zitat Gurunathan T, Mohanty S, Nayak SK (2015) A review of the recent developments in biocomposites based on natural fibres and their application perspectives. Composites A 77:1–25 Gurunathan T, Mohanty S, Nayak SK (2015) A review of the recent developments in biocomposites based on natural fibres and their application perspectives. Composites A 77:1–25
Zurück zum Zitat Huang D, Niu LL, Li J, Du JJ, Wei Y, Hu YC, Lian XJ, Chen WY, Wang KQ (2018) Reinforced chitosan membranes by microspheres for guided bone regeneration. J Mech Behav Biomed 81:195–201 Huang D, Niu LL, Li J, Du JJ, Wei Y, Hu YC, Lian XJ, Chen WY, Wang KQ (2018) Reinforced chitosan membranes by microspheres for guided bone regeneration. J Mech Behav Biomed 81:195–201
Zurück zum Zitat Jiang LY, Li YB, Wang XJ, Zhang L, Wen JQ, Gong M (2008) Preparation and properties of nano-hydroxyapatite/chitosan/carboxymethy cellulose composite scaffold. Carbohyd Polym 74:680–684 Jiang LY, Li YB, Wang XJ, Zhang L, Wen JQ, Gong M (2008) Preparation and properties of nano-hydroxyapatite/chitosan/carboxymethy cellulose composite scaffold. Carbohyd Polym 74:680–684
Zurück zum Zitat Jiang LY, Li YB, Xiong CD (2009) A novel composite membrane of chitosan-carboxymethyl cellulose polyelectrolyte complex membrane filled with nano-hydroxyapatite I. Preparation and properties. J Mater Sci 16:65 Jiang LY, Li YB, Xiong CD (2009) A novel composite membrane of chitosan-carboxymethyl cellulose polyelectrolyte complex membrane filled with nano-hydroxyapatite I. Preparation and properties. J Mater Sci 16:65
Zurück zum Zitat Jiang T, Carbone EJ, Lo KWH, Laurencin CT (2015) Electrospinning of polymer nanofibers for tissue regeneration. Prog Polym Sci 46:1–24 Jiang T, Carbone EJ, Lo KWH, Laurencin CT (2015) Electrospinning of polymer nanofibers for tissue regeneration. Prog Polym Sci 46:1–24
Zurück zum Zitat Jiang LY, Li Y, Xiong CD, Su SP, Ding HJ (2017) Preparation and properties of bamboo fiber/nano-hydroxyapatite/poly(lactic-co-glycolic) composite scaffold for bone tissue engineering. ACS Appl Mater Int 9:4890–4897 Jiang LY, Li Y, Xiong CD, Su SP, Ding HJ (2017) Preparation and properties of bamboo fiber/nano-hydroxyapatite/poly(lactic-co-glycolic) composite scaffold for bone tissue engineering. ACS Appl Mater Int 9:4890–4897
Zurück zum Zitat Jiang LY, Ma BL, Li Y, Ding HJ, Su SP, Xiong CD (2019) Effect of bamboo fiber on the degradation behavior and in vitro cytocompatibility of the nano-hydroxyapatite/poly(lactide-co-glycolide) (n-HA/PLGA) composite. Cellulose 26:1099–1110 Jiang LY, Ma BL, Li Y, Ding HJ, Su SP, Xiong CD (2019) Effect of bamboo fiber on the degradation behavior and in vitro cytocompatibility of the nano-hydroxyapatite/poly(lactide-co-glycolide) (n-HA/PLGA) composite. Cellulose 26:1099–1110
Zurück zum Zitat Khalil HPSA, Bhat IUH, Jawaid M, Zaidon A, Hermawan D, Hadi YS (2012) Bamboo fibre reinforced biocomposites: a review. Mater Des 42:353–368 Khalil HPSA, Bhat IUH, Jawaid M, Zaidon A, Hermawan D, Hadi YS (2012) Bamboo fibre reinforced biocomposites: a review. Mater Des 42:353–368
Zurück zum Zitat Kokubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 27:2907–2915 PubMed Kokubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 27:2907–2915 PubMed
Zurück zum Zitat Kumar JP, Lakshmi L, Jyothsna V, Balaji DR, Saravanan S, Moorthi A, Selvamurugan N (2014) Synthesis and characterization of diopside particles and their suitability along with chitosan matrix for bone tissue engineering in vitro and in vivo. J Biomed Nanotechnol 10:970–981 PubMed Kumar JP, Lakshmi L, Jyothsna V, Balaji DR, Saravanan S, Moorthi A, Selvamurugan N (2014) Synthesis and characterization of diopside particles and their suitability along with chitosan matrix for bone tissue engineering in vitro and in vivo. J Biomed Nanotechnol 10:970–981 PubMed
Zurück zum Zitat Li WW, Ma GW, Brazile B, Li N, Dai W, Butler JR, Claude AA, Wertheim JA, Liao J, Wang B (2015) Investigating the potential of amnion-based scaffolds as a barrier membrane for guided bone regeneration. Langmuir 31:8642–8653 PubMed Li WW, Ma GW, Brazile B, Li N, Dai W, Butler JR, Claude AA, Wertheim JA, Liao J, Wang B (2015) Investigating the potential of amnion-based scaffolds as a barrier membrane for guided bone regeneration. Langmuir 31:8642–8653 PubMed
Zurück zum Zitat Li P, Jia Z, Wang Q, Tang Wang PM, Wang K, Fang J, Zhao C, Ren F, Ge X, Lu X (2018) A resilient and flexible chitosan/silk cryogel incorporated Ag/Sr co-doped nanoscale hydroxyapatite for osteoinductivity and antibacterial properties. J Mater Chem B 6:7427–7438 PubMed Li P, Jia Z, Wang Q, Tang Wang PM, Wang K, Fang J, Zhao C, Ren F, Ge X, Lu X (2018) A resilient and flexible chitosan/silk cryogel incorporated Ag/Sr co-doped nanoscale hydroxyapatite for osteoinductivity and antibacterial properties. J Mater Chem B 6:7427–7438 PubMed
Zurück zum Zitat Liu D, Song J, Anderson DP, Chang PR, Hua Y (2012) Bamboo fiber and its reinforced composites: structure and properties. Cellulose 19:1449–1480 Liu D, Song J, Anderson DP, Chang PR, Hua Y (2012) Bamboo fiber and its reinforced composites: structure and properties. Cellulose 19:1449–1480
Zurück zum Zitat Long HB, Wu ZQ, Dong QQ, Shen YT, Zhou WY, Luo Y, Zhang CQ, Dong XM (2019) Effect of polyethylene glycol on mechanical properties of bamboo fiber-reinforced polylactic acid composites. J Appl Polym Sci 136:47709 Long HB, Wu ZQ, Dong QQ, Shen YT, Zhou WY, Luo Y, Zhang CQ, Dong XM (2019) Effect of polyethylene glycol on mechanical properties of bamboo fiber-reinforced polylactic acid composites. J Appl Polym Sci 136:47709
Zurück zum Zitat Manchineella S, Thrivikraman G, Basu B, Govindaraju T (2016) Surface-functionalized silk fibroin films as a platform to guide neuron-like differentiation of human mesenchymal stem cells. ACS Appl Mater Interface 8:22849–22859 Manchineella S, Thrivikraman G, Basu B, Govindaraju T (2016) Surface-functionalized silk fibroin films as a platform to guide neuron-like differentiation of human mesenchymal stem cells. ACS Appl Mater Interface 8:22849–22859
Zurück zum Zitat Norowski PA, Fujiwara T, Clem WC, Adatrow PC, Eckstein EC, Haggard WO, Bumgardner JD (2015) Novel naturally crosslinked electrospun nanofibrous chitosan mats for guided bone regeneration membranes: material characterization and cytocompatibility. J Tissue Eng Regen M 9:577–583 Norowski PA, Fujiwara T, Clem WC, Adatrow PC, Eckstein EC, Haggard WO, Bumgardner JD (2015) Novel naturally crosslinked electrospun nanofibrous chitosan mats for guided bone regeneration membranes: material characterization and cytocompatibility. J Tissue Eng Regen M 9:577–583
Zurück zum Zitat Palazzo B, Izzo D, Scalera F, Cancelli AN, Gervaso F (2014) Bio-hybrid scaffolds for bone tissue engineering: nano-hydroxyapatite/chitosan composites. Key Eng Mater 631:300–305 Palazzo B, Izzo D, Scalera F, Cancelli AN, Gervaso F (2014) Bio-hybrid scaffolds for bone tissue engineering: nano-hydroxyapatite/chitosan composites. Key Eng Mater 631:300–305
Zurück zum Zitat Park SM, Kim HS (2017) Preparation of acetylated chitosan/carbonated hydroxyapatite composite barriers for guided bone regeneration. Macromol Res 25:158–164 Park SM, Kim HS (2017) Preparation of acetylated chitosan/carbonated hydroxyapatite composite barriers for guided bone regeneration. Macromol Res 25:158–164
Zurück zum Zitat Phuong HAL, Ayob NAI, Blanford CF, Rawi NFM, Szekely G (2019) Nonwoven membrane supports from renewable resources: bamboo fiber reinforced poly(lactic acid) composites. ACS Sustain Chem Eng 7:11885–11893 Phuong HAL, Ayob NAI, Blanford CF, Rawi NFM, Szekely G (2019) Nonwoven membrane supports from renewable resources: bamboo fiber reinforced poly(lactic acid) composites. ACS Sustain Chem Eng 7:11885–11893
Zurück zum Zitat Tamburaci S, Tihminlioglu F (2017) Diatomite reinforced chitosan composite membrane as potential scaffold for guided bone regeneration. Mat Sci Eng C-Mater 80:222–231 Tamburaci S, Tihminlioglu F (2017) Diatomite reinforced chitosan composite membrane as potential scaffold for guided bone regeneration. Mat Sci Eng C-Mater 80:222–231
Zurück zum Zitat Tu Y, Chen C, Li YB, Hou Y, Huang M, Zhang L (2017) Fabrication of nano-hydroxyapatite/chitosan membrane with asymmetric structure and its applications in guided bone regeneration. Bio-Med Mater Eng 28:223–233 Tu Y, Chen C, Li YB, Hou Y, Huang M, Zhang L (2017) Fabrication of nano-hydroxyapatite/chitosan membrane with asymmetric structure and its applications in guided bone regeneration. Bio-Med Mater Eng 28:223–233
Zurück zum Zitat Wang ZL, Xu Y, Wang Y, Ito Y, Zhang PB, Chen XS (2016) Enhanced in vitro mineralization and in vivo osteogenesis of composite scaffolds through controlled surface grafting of L-lactic acid oligomer on nano-hydroxyapatite. Biomacromol 17:818–829 Wang ZL, Xu Y, Wang Y, Ito Y, Zhang PB, Chen XS (2016) Enhanced in vitro mineralization and in vivo osteogenesis of composite scaffolds through controlled surface grafting of L-lactic acid oligomer on nano-hydroxyapatite. Biomacromol 17:818–829
Zurück zum Zitat Wu ZY, Tang TT, Guo H, Tang SC, Niu YF, Zhang J, Zhang WJ, Ma R, Su JC, Liu CS, Wei J (2014) In vitro degradability, bioactivity and cell responses to mesoporous magnesium silicate for the induction of bone regeneration. Colloid Surf B 120:38–46 Wu ZY, Tang TT, Guo H, Tang SC, Niu YF, Zhang J, Zhang WJ, Ma R, Su JC, Liu CS, Wei J (2014) In vitro degradability, bioactivity and cell responses to mesoporous magnesium silicate for the induction of bone regeneration. Colloid Surf B 120:38–46
Zurück zum Zitat Xiang WY, Li SB, Li K, Li N (2016) Preparation of bamboo fibers adsorbent modified by citric acid. Chin J Environ Eng 10:5542–5548 Xiang WY, Li SB, Li K, Li N (2016) Preparation of bamboo fibers adsorbent modified by citric acid. Chin J Environ Eng 10:5542–5548
Zurück zum Zitat Xu LJ, Jiang LY, Xiong CD, Jiang LX (2014) Effect of different synthesis conditions on the microstructure, crystallinity and solubility of Mg-substituted hydroxyapatite nanopowder. Adv Powder Technol 25:1142–1146 Xu LJ, Jiang LY, Xiong CD, Jiang LX (2014) Effect of different synthesis conditions on the microstructure, crystallinity and solubility of Mg-substituted hydroxyapatite nanopowder. Adv Powder Technol 25:1142–1146
Zurück zum Zitat Ye HL, Zhu JJ, Deng D, Jin SE, Li JD, Man Y (2019) Enhanced osteogenesis and angiogenesis by PCL/chitosan/Sr-doped calcium phosphate electrospun nanocomposite membrane for guided bone regeneration. J Biomater Sci-Polym E 30:1505–1522 Ye HL, Zhu JJ, Deng D, Jin SE, Li JD, Man Y (2019) Enhanced osteogenesis and angiogenesis by PCL/chitosan/Sr-doped calcium phosphate electrospun nanocomposite membrane for guided bone regeneration. J Biomater Sci-Polym E 30:1505–1522
Zurück zum Zitat Zhang Y, Wu H, Qiu Y (2010) Morphology and properties of hybrid composites based on polypropylene/polylactic acid blend and bamboo fiber. Bioresour Technol 101:7944–7950 Zhang Y, Wu H, Qiu Y (2010) Morphology and properties of hybrid composites based on polypropylene/polylactic acid blend and bamboo fiber. Bioresour Technol 101:7944–7950
Zurück zum Zitat Zhang Y, Zhang X, Shi B, Miron RJ (2013) Membranes for guided tissue and bone regeneration. Ann Oral Maxillofac Surg 1:10 Zhang Y, Zhang X, Shi B, Miron RJ (2013) Membranes for guided tissue and bone regeneration. Ann Oral Maxillofac Surg 1:10
Zurück zum Zitat Zhou T, Liu X, Sui BY, Liu C, Mo XM, Sun J (2017a) Development of fish collagen/bioactive glass/chitosan composite nanofibers as a GTR/GBR membrane for inducing periodontal tissue regeneration. Biomed Mater 12:055004 PubMed Zhou T, Liu X, Sui BY, Liu C, Mo XM, Sun J (2017a) Development of fish collagen/bioactive glass/chitosan composite nanofibers as a GTR/GBR membrane for inducing periodontal tissue regeneration. Biomed Mater 12:055004 PubMed
Zurück zum Zitat Zhou YS, Dong Q, Yang HJ, Liu X, Yin XZ, Tao YZ, Bai ZK, Xu WL (2017b) Photocrosslinked maleilated chitosan/ methacrylated poly (vinyl alcohol) bicomponent nanofibrous scaffolds for use as potential wound dressings. Carbohyd Polym 168:220–226 Zhou YS, Dong Q, Yang HJ, Liu X, Yin XZ, Tao YZ, Bai ZK, Xu WL (2017b) Photocrosslinked maleilated chitosan/ methacrylated poly (vinyl alcohol) bicomponent nanofibrous scaffolds for use as potential wound dressings. Carbohyd Polym 168:220–226
Metadaten
Titel
Preparation and characterization of bamboo fiber/chitosan/nano-hydroxyapatite composite membrane by ionic crosslinking
verfasst von
Shuo Tang
Liuyun Jiang
Bingli Ma
Chunyan Tang
Yue Wen
Na Zhang
Yan Zhang
Shengpei Su
Publikationsdatum
09.04.2020
Verlag
Springer Netherlands
Erschienen in
Cellulose / Ausgabe 9/2020
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-020-03145-2

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