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Journal of Sol-Gel Science and Technology
Erschienen in: Journal of Sol-Gel Science and Technology 3/2018

24.07.2018 | Original Paper: Nano-structured materials (particles, fibers, colloids, composites, etc.)

Preparation of hierarchically mesoporous bioactive glass and immobilization of lysozyme

verfasst von: Ruiping Luo, Xinrui Zhou, Yi Xiu, Hongsu Wang

Erschienen in: Journal of Sol-Gel Science and Technology | Ausgabe 3/2018

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Abstract

Hierarchically mesoporous bioactive glass (HMBG) was prepared using pine pollen as the biological template for lysozyme immobilization via intermolecular interactions. Effects of the initial concentration of lysozyme, pH, stirring speed (rpm), and temperature on lysozyme adsorption were investigated. The results showed that HMBG could immobilize lysozyme efficiently, and a maximum adsorption capacity of 648 ± 5.1 mg g−1 was achieved at the optimum immobilization conditions. Moreover, it was found that the adsorption process fit well with the Langmuir isothermal model. Morphology and pore structure of prepared HMBG were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray diffraction (XRD), and nitrogen adsorption–desorption measurements. Lysozyme immobilization was verified by Fourier transform infrared (FTIR) spectroscopy. In addition, no change in the secondary structure of lysozyme during the adsorption/desorption process was demonstrated by UV–visible spectroscopy (UV–Vis), molecular fluorescence spectrometry (MFS), and circular dichroism (CD) spectroscopy. Higher stability and better reusability of lysozyme–HMBG than those of alternatives were also shown.
Vorheriger Artikel Acid acting as redispersing agent to form stable colloids from photoactive crystalline aqueous sol–gel TiO2 powder
Nächster Artikel Study on the synthesis of Fe2O3-, MgO-cored glass by sol-gel method and their application in agricultural chemistry

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Literatur
1.
Brady D, Jordaan J (2009) Advances in enzyme immobilisation. Biotechnol Lett 31:1639–1650CrossRef
2.
Shirahama H, Lyklema J, Norde W (1990) Comparative protein adsorption in model systems. J Colloid Interface Sci 139:177–187CrossRef
3.
Dan H, Dong X, Lu X, Ding Y (2017) Facile route to synthesize mesoporous SBA-15 rods with different sizes for lysozyme immobilization. J Sol–Gel Sci Technol 81:782–790CrossRef
4.
Bayazidi P, Almasi H, Asl AK (2018) Immobilization of lysozyme on bacterial cellulose nanofibers: characteristics, antimicrobial activity and morphological properties. Int J Biol Macromol 107:2544–2551CrossRef
5.
Elfiqi A, Kim JH, Kim HW (2015) Osteoinductive fibrous scaffolds of biopolymer/mesoporous bioactive glass nanocarriers with excellent bioactivity and long-term delivery of osteogenic drug. Acs Appl Mater Interfaces 7:1140–1152CrossRef
6.
Sheldon RA (2007) Enzyme immobilization: the quest for optimum performance. Adv Synth Catal 349:1289–1307CrossRef
7.
Fried DI, Brieler FJ, Fröba M (2013) Designing inorganic porous materials for enzyme adsorption and applications in biocatalysis. Chem Cat Chem 5:862–884
8.
Lv Z, Luo R, Xi L, Chen Y, Wang H (2017) An amperometric acetylcholinesterase sensor based on the bio-templated synthesis of hierarchical mesoporous bioactive glass microspheres. J Electron Mater 46:6578–6587CrossRef
9.
Park M, Park SS, Selvaraj M, Zhao D, Ha C-S (2009) Hydrophobic mesoporous materials for immobilization of enzymes. Microporous Mesoporous Mater 124:76–83CrossRef
10.
Xiao QG, Tao X, Zou HK, Chen JF (2008) Comparative study of solid silica nanoparticles and hollow silica nanoparticles for the immobilization of lysozyme. Chem Eng J 137:38–44CrossRef
11.
Hench LL, Splinter RJ, Allen WC, Greenlee TK (1971) Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res A 5:117–141CrossRef
12.
Wang H, Gao X, Wang Y, Tang J, Sun C, Deng X, Niu X (2012) Bio-templated synthesis of mesoporous bioactive glass with a hierarchical pore structure. Mater Lett 76:237–239CrossRef
13.
Yan P, Wang J, Ou J, Li Z, Lei Z, Yang S (2010) Synthesis and characterization of three-dimensional ordered mesoporous–macroporous bioactive glass. Mater Lett 64:2544–2547CrossRef
14.
Lei B, Chen X, Han X, Zhou J (2012) Versatile fabrication of nanoscale sol–gel bioactive glass particles for efficient bone tissue regeneration. J Mater Chem 22:16906–16913CrossRef
15.
Hu Q, Li Y, Miao G, Zhao N, Chen X (2014) Size control and biological properties of monodispersed mesoporous bioactive glass sub-micron spheres. RSC Adv 4:22678–22687CrossRef
16.
Yang S, Wang J, Tang L, Ao H, Tan H, Tang T, Liu C (2014) Mesoporous bioactive glass doped-poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) composite scaffolds with 3-dimensionally hierarchical pore networks for bone regeneration. Colloid Surf B 116:72–80CrossRef
17.
Wang D, Lin H, Jiang J, Han X, Guo W, Wu X, Jin Y, Qu F (2013) One-pot synthesis of magnetic, macro/mesoporous bioactive glasses for bone tissue engineering. Sci Technol Adv Mater 14:025004CrossRef
18.
Shi QH, Wang JF, Zhang JP, Fan J, Stucky GD (2010) Rapid-setting, mesoporous, bioactive glass cements that induce accelerated in vitro apatite formation. Adv Mater 18:1038–1042CrossRef
19.
Li X, Qu F, Li W, Lin H, Jin Y (2012) Synthesis of hierarchically porous bioactive glasses using natural plants as template for bone tissue regeneration. J Sol–Gel Sci Technol 63:416–424CrossRef
20.
He W, Min D, Zhang X, Zhang Y, Bi Z, Yue Y (2014) Hierarchically nanoporous bioactive glasses for high efficiency immobilization of enzymes. Adv Funct Mater 24:2206–2215CrossRef
21.
Bhattacharyya MS, Hiwale P, Piras, Medda M, Steri L, Piludu D, Ma X (2010) Lysozyme adsorption and release from ordered mesoporous materials. J Phys Chem C 114:19928–19934CrossRef
22.
Valletregí M, Romero AM, Ragel CV, Legeros RZ (1999) XRD, SEM-EDS, and FTIR studies of in vitro growth of an apatite-like layer on sol–gel glasses. J Biomed Mater Res 44:416CrossRef
23.
Karakisla M, Bayramoğlu G, Arıca MY (2008) Preparation of methacrylamide grafted and dye-ligand immobilized PET fibers: studies of adsorption and purification of lysozyme. J Appl Polym Sci 108:3313–3323CrossRef
24.
Smith SC, Ahmed F, Gutierrez KM, Rodrigues DF (2014) A comparative study of lysozyme adsorption with graphene, graphene oxide, and single-walled carbon nanotubes: Potential environmental applications. Chem Eng J 240:147–154CrossRef
25.
Sun J, Wu L, Chen J (2016) Efficient lysozyme adsorption on chitosan/hydroxyapatite hybrid membrane via in situ synthesis. Cellu 23:1–14CrossRef
26.
Karpovich DS, Blanchard GJ (1994) Direct measurement of the adsorption kinetics of alkanethiolate self-assembled monolayers on a microcrystalline gold surface. Langmuir 10:3315–3322CrossRef
27.
Areco MM, Dos SAM (2010) Copper, zinc, cadmium and lead biosorption by Gymnogongrus torulosus. Thermodynamics and kinetics studies. Colloid Surf B 81:620–628CrossRef
28.
Yang S, Wang Y, Xu M, He M, Zhang M, Ran D, Jia X (2013) Synthesis of modified chitosan-based molecularly imprinted polymers for adsorptive protein separation. Anal Methods 5:5471–5477CrossRef
29.
Zhu X, Zhang L, Fu A, Yuan H (2016) Efficient purification of lysozyme from egg white by 2-mercapto-5-benzimidazolesulfonic acid modified Fe3O4/Au nanoparticles. Mat Sci Eng C-Mater 59:213–217CrossRef
30.
Ahmad H, Okubo M, Kamatari YO, Minami H (2002) Structural conformation of biomolecules released from temperature-sensitive composite polymer particles—a study by circular dichroism. Colloid Polym Sci 280:310–315CrossRef
31.
Keiderling TA (2002) Protein and peptide secondary structure and conformational determination with vibrational circular dichroism. Curr Opin Chem Biol 6:682–688CrossRef
32.
Myer YP (1968) Conformation of cytochromes. II. Comparative study of circular dichroism spectra, optical rotatory dispersion, and absorption spectra of horse heart cytochrome c. J Biol Chem 243:2115–2122
33.
Deka JR, Saikia D, Lai YS, Tsai CH, Chang WC, Kao HM (2015) Roles of nanostructures and carboxylic acid functionalization of ordered cubic mesoporous silicas in lysozyme immobilization. Micro Mesopor Mat 213:150–160CrossRef
34.
Asuri P, Bale SS, Pangule RC, Shah DA, And RSK, Dordick JS (2016) Structure, function, and stability of enzymes covalently attached to single-walled carbon nanotubes. Langmuir 23:12318–12321CrossRef
Metadaten
Titel
Preparation of hierarchically mesoporous bioactive glass and immobilization of lysozyme
verfasst von
Ruiping Luo
Xinrui Zhou
Yi Xiu
Hongsu Wang
Publikationsdatum
24.07.2018
Verlag
Springer US
Erschienen in
Journal of Sol-Gel Science and Technology / Ausgabe 3/2018
Print ISSN: 0928-0707
Elektronische ISSN: 1573-4846
DOI
https://doi.org/10.1007/s10971-018-4753-4

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