Abstract
This chapter describes a simple system for cryopreservation of avian spermatozoa as pellets, formed by dropping volumes of sperm suspension directly into liquid nitrogen with dimethylacetamide as cryoprotectant. The method originates from the group at the Research Institute of Farm Animal Breeding and Genetics at St. Petersburg-Pushkin and is described here for chicken spermatozoa, but has also been adapted successfully for other species, such as Houbara bustards and pheasants.
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References
Polge, C. (1951) Functional survival of fowl spermatozoa after freezing at −70°C. Nature 167, 949–950.
Wishart, G. J. (1995) Cryopreservation of avian spermatozoa. In: Cryopreservation and Freeze-Drying Protocols, (Day, J. G. and McLellan, M. R., eds.), Humana Press, Totowa, NJ, pp. 167–177.
Tselutin, K., Seigneurin, F., and Blesbois, E. (1999) Comparison of cryoprotectants and methods of cryopreservation of fowl spermatozoa. Poult. Sci. 78, 586–590.
Hartley, P., Dawson, R., Lindsay, C., McCormick, P., and Wishart, G. (1999) Cryopreservation of Houbara semen: a pilot study. Zoo Biol. 18, 147–152.
St. Jalme, M., Lecoq, R., Seigneurin, F., Blesbois, E., and Plouzeau, H. (2003) Cryopreservation of semen from endangered pheasants: the first step towards a cryobank for endangered species. Theriogenology 59, 875–888.
Gee, G. F., Bertschinger, H., Donoghue, A. M., Blanco, J., and Soley, J. (2004) Reproduction in non-domestic birds: physiology, semen collection, artificial insemination and cryopreservation. Avian Poult. Biol. Rev. 15, 47–101.
Knowles-Brown, A. and Wishart, G. J. (2001) Progeny from cryopreserved golden eagle spermatozoa. Avian Poult. Biol. Rev. 12, 201.
Wishart, G. J. (2003) The cryopreservation of germplasm in domestic and nondomestic birds. In: Cryobanking the Genetic Resource, (Watson, P. F. and Holt, W. V., eds.), Taylor and Francis, London and New York, pp. 180–200.
Surai, P. F. and Wishart, G. J. (1996) Poultry artificial insemination technology in the countries of the former USSR. Worlds Poult. Sci. J. 52, 27–43.
Tselutin, K., Narubina, L., Mavrodina, T., and Tur, B. (1995) Cryopreservation of poultry semen. Br. Poult. Sci. 36, 805–811.
Pisenti, J. M., Delany, M. E., Taylor, R. L. et al. (1999) Avian genetic resources at risk. Report No. 20. University of California Division of Agriculture and Natural Resources Genetic Resources Conservation Program. Davis, CA, p. 120.
ERFP. (2003) Guidelines for the Constitution of National Cryopreservation Programmes for Farm Animals. Publication 1 of the European Focal Point on Animal Genetic Resources. (Hiemstra, S. J., ed.).
Lake, P. E. and Stewart, J. M. (1978) Artificial insemination in poultry. Bulletin 213 of the Ministry of Agriculture, Fisheries and Food. Her Majesty’s Stationary Office, London, UK.
Lake, P. E. and Ravie, O. (1984) An exploration of cryoprotective compounds for fowl spermatozoa. Br. Poult. Sci. 25, 145–150.
Lake, P. E. (1986) The history and future of the cryopreservation of avian germ plasm. Poult. Sci. 65, 1–15.
Donoghue, A. M. and Wishart, G. J. (2000) Storage of poultry semen. Anim. Reprod. Sci. 62, 213–232.
Blesbois, E. and Labbe, C. (2003) Main improvements in semen and embryo cryopreservation for fish and fowl. In: Workshop on Cryopreservation of Animal Resources in Europe, (Planchenault, D., ed.), Paris, France, pp. 55–65.
Buss, E. G. (1993) Cryopreservation of rooster sperm. Poult. Sci. 72, 944–954.
Wishart, G. J. (1997) Quantitative aspects of sperm:egg interaction in chickens and turkeys. Anim. Reprod. Sci. 48, 81–92.
Fox, N. (1995) Understanding the Birds of Prey. Hancock House Publishers. Surrey, British Columbia.
Ansah, G. A. and Buckland, R. B. (1983) Eight generations of selection for duration of fertility of frozen-thawed semen in the chicken. Poult. Sci. 62, 1529–1538.
McCormick, P. (1996) International Foundation for the Conservation and Development of Wildlife: Review 1993–1996.
Blanco, J. M., Gee, G., Wildt, D. E., and Donoghue, A. M. (2002) Producing progeny from endangered birds of prey: treatment of urine-contaminated semen and a novel intramagnal insemination approach. J. Zoo Wildl. Med. 33, 1–7.
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Wishart, G.J. (2007). Cryopreservation of Avian Spermatozoa. In: Day, J.G., Stacey, G.N. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology™, vol 368. Humana Press. https://doi.org/10.1007/978-1-59745-362-2_15
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DOI: https://doi.org/10.1007/978-1-59745-362-2_15
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