nach oben

Experiments in Fluids

Erschienen in:

01.10.2021 | Research Article

Rhodamine-based caged dye for aqueous MTV with green lasers

verfasst von: Charles Fort, Philippe M. Bardet

Erschienen in: Experiments in Fluids | Ausgabe 10/2021

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config

KI-gestützte Suche

Aus

Abstract

Molecular tagging velocimetry (MTV) is a nonintrusive optical diagnostic with typically three variants in liquids distinguished by the dye that they rely on: phosphorescent, photochromic, or caged. The latter scheme, initially called photo-activated nonintrusive tracking of molecular motion (PHANTOMM), required both a UV and a dedicated blue laser. Here, we demonstrate caged-dye-based MTV with pulsed UV and green lasers for the first time, simplifying its deployment in most experimental fluid dynamics laboratories.

Vorheriger Artikel Non-axisymmetric complete flow conditioning gauzes

Nächster Artikel An experimental approach to analyze aerosol and splatter formations due to a dental procedure

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Biage M, Campos JCC (2003) Visualization study and quantitative velocity measurements in turbulent taylor-couette flow by phantomm flow tagging: a description of the transition to turbulence. J Braz Soc Mech Sci 25(4):378–390CrossRef

Bonin K, Smelser A, Moreno NS, Holzwarth G, Wang K, Levy P, Vidi P-A (2018) Structured illumination to spatially map chromatin motions. J Biomed Opt 23(5):056007CrossRef

Dussaud AD, Troian SM, Harris SR (1998) Fluorescence visualization of a convective instability which modulates the spreading of volatile surface films. Phys Fluids 10(7):1588–1596CrossRef

Fort C, Bardet PM (2021) Efficient photobleaching of rhodamine 6G by a single UV pulse. Appl Opt 60(22):6342–6350CrossRef

Fort C, André MA, Bardet PM (2020a) Development of long distance 2D micro-molecular tagging velocimetry (\(\mu \)MTV) to measure wall shear stress. In: AIAA Scitech Forum, p 1274

Fort C, André MA, Pazhand H, Bardet PM (2020b) Talbot-effect structured illumination: pattern generation and application to long-distance \(\upmu \)-MTV. Exp Fluids 61(2):40CrossRef

Gee KR, Weinberg ES, Kozlowski DJ (2001) Caged Q-rhodamine dextran: a new photoactivated fluorescent tracer. Bioorg Med Chem Lett 11(16):2181–2183CrossRef

Guilkey JE, Gee KR, McMurtry PA, Klewicki JC (1996) Use of caged fluorescent dyes for the study of turbulent passive scalar mixing. Exp Fluids 21(4):237–242CrossRef

Harris SR (1999) Quantitative measurements in a lid-driven, cylindrical cavity using the PHANTOMM flow-tagging technique. PhD thesis, Princeton University

Hauke S, von Appen A, Quidwai T, Ries J, Wombacher R (2017) Specific protein labeling with caged fluorophores for dual-color imaging and super-resolution microscopy in living cells. Chem Sci 8(1):559–566CrossRef

Jasuja R, Keyoung J, Reid GP, Trentham DR, Khan S (1999) Chemotactic responses of Escherichia coli to small jumps of photoreleased l-aspartate. Biophys J 76(3):1706–1719CrossRef

Johnson ID (2006) Practical considerations in the selection and application of fluorescent probes. Handbook of biological confocal microscopy. Springer, Boston, pp 353–367CrossRef

Klán P, Solomek T, Bochet CG, Blanc A, Givens R, Rubina M, Popik V, Kostikov A, Wirz J (2012) Photoremovable protecting groups in chemistry and biology: reaction mechanisms and efficacy. Chem Rev 113(1):119–191CrossRef

Koochesfahani MM, Nocera DG (2007) Molecular tagging velocimetry. In: Tropea C, Yarin A, Foss J (eds) Handbook of experimental fluid dynamics, Chap. 5.4. Springer, Berlin, pp 362–382

Kozma E, Kele P (2019) Fluorogenic probes for super-resolution microscopy. Organ Biomol Chem 17(2):215–233CrossRef

Lempert WR, Ronney P, Magee K, Gee KR, Haugland RP (1995) Flow tagging velocimetry in incompressible flow using photo-activated nonintrusive tracking of molecular motion (phantomm). Exp Fluids 18(4):249–257CrossRef

Lempert WR, Lee D, Harris SR, Miles RB, Gee KR (1998) Synthesis and characterization of new caged dye velocimetry tracers for microgravity droplet diagnostics 27: 512

Li W-H, Zheng G (2012) Photoactivatable fluorophores and techniques for biological imaging applications. Photochem Photobiol Sci 11(3):460–471MathSciNetCrossRef

Lohse S, Kohnen BT, Janasek D, Dittrich PS, Franzke J, Agar DW (2008) A novel method for determining residence time distribution in intricately structured microreactors. Lab Chip 8(3):431–438CrossRef

Mitchison TJ, Sawin KE, Theriot JA, Gee K, Mallavarapu A (1998) [4] caged fluorescent probes. Methods Enzymol 291:63–78CrossRef

Molho JI, Herr AE, Mosier BP, Santiago JG, Kenny TW, Brennen RA, Gordon GB, Mohammadi B (2001) Optimization of turn geometries for microchip electrophoresis. Anal Chem 73(6):1350–1360CrossRef

Park JS, Kim HJ, Kihm KD (2001) Molecular tagging fluorescence velocimetry (mtfv) for lagrangian flow field mapping inside evaporating meniscus: potential use for microscale applications. J Flow Visual Image Process 8:177–187

Park JS, McCarty C, Kihm KD, Pratt DM (2000) Lagrangian flow mapping of heated capillary pore and thin film using molecular fluorescence velocimetry (MFV). J Heat Transf 122(3):423CrossRef

Paul PH, Garguilo MG, Rakestraw DJ (1998) Imaging of pressure-and electrokinetically driven flows through open capillaries. Anal Chem 70(13):2459–2467CrossRef

Payumo AY, Walker WJ, McQuade LE, Yamazoe S, Chen JK (2015) Optochemical dissection of t-box gene-dependent medial floor plate development. ACS Chem Biol 10(6):1466–1475CrossRef

Pelliccioli AP, Wirz J (2002) Photoremovable protecting groups: reaction mechanisms and applications. Photochem Photobiol Sci 1(7):441–458CrossRef

Roetmann K, Schmunk W, Garbe CS, Beushausen V (2008) Micro-flow analysis by molecular tagging velocimetry and planar Raman-scattering. Exp Fluids 44(3):419–430CrossRef

Ross D, Johnson TJ, Locascio LE (2001) Imaging of electroosmotic flow in plastic microchannels. Anal Chem 73(11):2509–2515CrossRef

Sawin KE, Mitchison TJ (1999) Photoactivation of fluorescence as a probe for cytoskeletal dynamics. In: Watson W (ed) Mitosis and cell motility, in Fluorescent and luminescent probes for biological activity. Academic Press, San Diego, USA, pp 613–627CrossRef

Schuster BS, Allan DB, Kays JC, Hanes J, Leheny RL (2017) Photoactivatable fluorescent probes reveal heterogeneous nanoparticle permeation through biological gels at multiple scales. J Control Release 260:124–133CrossRef

Shelby JP, Chiu DT (2003) Mapping fast flows over micrometer-length scales using flow-tagging velocimetry and single-molecule detection. Anal Chem 75(6):1387–1392CrossRef

Sinton D, Erickson D, Li D (2002) Photo-injection based sample design and electroosmotic transport in microchannels. J Micromech Microeng 12(6):898CrossRef

Wysocki LM, Grimm JB, Tkachuk AN, Brown TA, Betzig E, Lavis LD (2011) Facile and general synthesis of photoactivatable xanthene dyes. Angew Chem Int Edn 50(47):11206–11209CrossRef

Xia P, Bungay PM, Gibson CC, Kovbasnjuk ON, Spring KR (1998) Diffusion coefficients in the lateral intercellular spaces of madin-darby canine kidney cell epithelium determined with caged compounds. Biophys J 74(6):3302–3312CrossRef

Zhao Y, Zheng Q, Dakin K, Xu K, Martinez ML, Li W-H (2004) New caged coumarin fluorophores with extraordinary uncaging cross sections suitable for biological imaging applications. J Am Chem Soc 126(14):4653–4663CrossRef

Titel: Rhodamine-based caged dye for aqueous MTV with green lasers
verfasst von: Charles Fort
Philippe M. Bardet
Publikationsdatum: 01.10.2021
Verlag: Springer Berlin Heidelberg
Erschienen in: Experiments in Fluids / Ausgabe 10/2021
Print ISSN: 0723-4864
Elektronische ISSN: 1432-1114
DOI: https://doi.org/10.1007/s00348-021-03303-7

Premium Partner

Marktübersichten

Die im Laufe eines Jahres in der „adhäsion“ veröffentlichten Marktübersichten helfen Anwendern verschiedenster Branchen, sich einen gezielten Überblick über Lieferantenangebote zu verschaffen.

Zur Marktübersicht

Springer Professional

Abstract

Bitte loggen Sie sich ein, um Zugang zu Ihrer Lizenz zu erhalten.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Weitere Artikel der Ausgabe 10/2021

Experimental investigation of the transonic buffet cycle on a supercritical airfoil

Effect of leading-edge protuberances on unsteady airfoil performance at low Reynolds number

An experimental approach to analyze aerosol and splatter formations due to a dental procedure

Controlled generation of single microbubbles

Measurement of dynamic wetting using phase-shifting imaging ellipsometer: comparison of pure solvent and nanoparticle suspension on film thickness profile, apparent contact angle, and precursor film length

FLEET velocimetry measurements in the ONR-UTA arc-jet wind tunnel

Premium Partner

Marktübersichten