Scale-Up of Liquid Chromatography using General Rate Models

One of the critical factors for a successful scale-up of liquid chromatography using the rates models is accurate parameter estimations. Three types of parameter are needed to carry out model calculations using the rate models. Isotherm parameters, the particle porosity and the bed void volume fraction are important to the accuracy of model calculations. Physical dimensions of the column are equally important, but they can be easily and precisely measured. Less important parameters are the mass transfer coefficients, which usually do not affect the general location of an elution peak. They affect the shape of the peak. However, such an influence is not very sensitive to the fluctuation of the mass transfer coefficients. Thus, parameter estimation of these coefficients does not have to be very stringent.