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Erschienen in:

2015 | Online First | Buchkapitel

Sendai Virus-Based Reprogramming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton’s Jelly into Induced Pluripotent Stem Cells

verfasst von : Cristian Miere, Liani Devito, Dusko Ilic

Erschienen in: Methods in Molecular Biology™

Verlag: Springer New York

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Abstract

In an attempt to bring pluripotent stem cell biology closer to reaching its full potential, many groups have focused on improving reprogramming protocols over the past several years. The episomal modified Sendai virus-based vector has emerged as one of the most practical ones. Here we describe reprogramming of mesenchymal stromal/stem cells (MSC) derived from umbilical cord Wharton’s Jelly into induced pluripotent stem cells (iPSC) using genome non-integrating Sendai virus-based vectors. The detailed protocols of iPSC colony cryopreservation (vitrification) and adaption to feeder-free culture conditions are also included.

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Titel: Sendai Virus-Based Reprogramming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton’s Jelly into Induced Pluripotent Stem Cells
verfasst von: Cristian Miere
Liani Devito
Dusko Ilic
Verlag: Springer New York
DOI: https://doi.org/10.1007/7651_2014_163

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Abstract

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