Weitere Artikel dieser Ausgabe durch Wischen aufrufen
The online version of this article (https://doi.org/10.1007/s10570-019-02285-4) contains supplementary material, which is available to authorized users.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Cellulose-binding modules (CBMs) are non-catalytic domains typically occurring in glycoside hydrolases. Their specific interaction with diverse polysaccharides assists hydrolysis by the catalytic subunits. In this work, we have exploited the interactions between a CBM from family 3 (CBM3) and cell wall polysaccharides to alter the structure and mechanical properties of cellulose microfibrils from BY-2 tobacco cell suspension cultures. A CBM3 from Clostridium thermocellum was overexpressed in the cells using Agrobacterium-mediated transformation. Water suspensions of cellulose microfibrils were prepared by the removal of the non-cellulosic components of the primary cell walls, followed by mild disintegration using sonication. The morphology of the microfibrils was characterized by transmission electron microscopy and atomic force microscopy. These cellulose microfibrils were further hydrolyzed with 64 wt% sulfuric acid to produce cellulose nanocrystals (CNCs). The average length of CNCs prepared from the CBM3-transformed cells was 201 nm, higher than that from the wild-type cells (122 nm). In addition, the mechanical properties and deformation mechanism of nanopapers prepared from suspensions of cellulose microfibrils were investigated. The nanopapers obtained from the CBM3-transformed cells exhibited enhanced tensile strength and work of fracture, 40% and 128% higher than those prepared from wild-type tobacco cells, respectively.
Supplementary material 1 Cloning, transformation and culturing of cell suspension cultures. (DOCX 17 kb)10570_2019_2285_MOESM1_ESM.docx
Albersheim P, Darvill A, Roberts K, Sederoff R, Staehelin A (2010) Plant cell walls. Garland Science, Taylor & Francis Group LLC, New York CrossRef
Carpita NC, Gibeaut DM (1993) Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J 3:1–30. https://doi.org/10.1111/j.1365-313X.1993.tb00007.x CrossRef
Dufresne A, Cavaille JY, Vignon MR (1997) Mechanical behavior of sheets prepared from sugar beet cellulose microfibrils. J Appl Polym Sci 64:1185–1194. https://doi.org/10.1002/(SICI)1097-4628(19970509)64:6%3c1185:AID-APP19%3e3.0.CO;2-V CrossRef
Dufresne A, Dupeyre D, Vignon MR (2000) Cellulose microfibrils from potato tuber cells: processing and characterization of starch-cellulose microfibril composites. J Appl Polym Sci 76:2080–2092. https://doi.org/10.1002/(SICI)1097-4628(20000628)76:14%3c2080:AID-APP12%3e3.0.CO;2-U CrossRef
Fukuzumi H, Saito T, Isogai A (2013) Influence of TEMPO-oxidized cellulose nanofibril length on film properties. Carbohyd Polym 93:172–177. https://doi.org/10.1016/j.carbpol.2012.04.069 CrossRef
Levy I, Shani Z, Shoseyov O (2002b) Modification of polysaccharides and plant cell wall by endo-1,4-β-glucanase and cellulose-binding domains. Biomol Eng 19:17–30. https://doi.org/10.1016/S1389-0344(02)00007-2 CrossRef
McCann MC, Wells B, Roberts K (1990) Direct visualization of cross-links in the primary plant cell wall. J Cell Sci 96:323–334
Shani Z, Shpigel E, Roiz L, Goren R, Vinocur B, Tzfira T, Altman A, Shoseyov O (1999) Cellulose binding domain increases cellulose synthase activity in Acetobacter xylinum, and biomass of transgenic plants. In: Altman A, Ziv M, Izhar S (eds) Plant biotechnology and in vitro biology in the 21st century. Springer, Dordrecht, pp 213–218. https://doi.org/10.1007/978-94-011-4661-6_50 CrossRef
Somerville C (2006) Cellulose synthesis in higher plants. Annu Rev Cell Dev Biol 22:53–78. https://doi.org/10.1146/annurev.cellbio.22.022206.160206 CrossRef
Tormo J, Lamed R, Chirino AJ, Morag E, Bayer EA, Shoham Y, Steitz TA (1996) Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. EMBO J 15:5739–5751 CrossRef
Varanasi S, Henzel L, Sharman S, Batchelor W, Garnier G (2018) Producing nanofibres from carrots with a chemical-free process. Carbohydr Polym 184:307–314 CrossRef
- Stronger cellulose microfibril network structure through the expression of cellulose-binding modules in plant primary cell walls
- Springer Netherlands
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
Systemische Notwendigkeit zur Weiterentwicklung von Hybridnetzen