106. Study on the Application of a Thermotolerant Saccharomyces cerevisiae in the Production of Bioethanol

Bioethanol has attracted extensive attention because of its advantages response to energy crisis and environment pollution. However, the ethanol production could be decreased due to the high temperature created by the exothermic reactions during the fermentation process of bioethanol. In this study, the thermotolerant Saccharomyces cerevisiae strain constructed in our precious work was applied to the very high gravity fermentation (VHG) process for the production of bio-ethanol. The ethanol production of F3 and the control strain AY12 via different fermentation techniques were tested. Our results illustrate that the F3 strain exhibits higher ethanol production compared to the strain AY12 in different temperatures, and that the fermentation technique of simultaneous saccharification and fermentation (SSF) possesses the predominance among the four. The orthogonal test was also performed to optimize the fermentation conditions. Finally, the ratio of nutriment and water 1:2.6, inoculum size 25 ml, fermentation period 56 h were identified as the optimal fermentation conditions and the ethanol production was 12.2 % (v/v).