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Journal of Materials Science
Erschienen in: Journal of Materials Science 12/2016

24.03.2016 | Original Paper

Surface-imprinted polymer coating l-cysteine-capped ZnS quantum dots for target protein specific recognition

verfasst von: Xin Zhang, Shu Yang, Liquan Sun, Aiqin Luo

Erschienen in: Journal of Materials Science | Ausgabe 12/2016

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Abstract

This paper demonstrated a new method for preparing fluorescent molecularly imprinted polymer (MIP) for specific recognition of a target protein. The MIP-based fluorescent receptor was developed by coating MIP layer on the surface of l-cysteine modified Mn2+-doped ZnS quantum dots (QDs) using the surface molecular imprinting process. These MIP-QDs composites demonstrated fast adsorption kinetics, high stability, and good dispersibility in aqueous media. Since the fluorescence quenching of MIP-QDs composites is proportional to the concentration of the lysozyme, the MIP-based fluorescent receptor was successfully applied to the direct fluorescence quantification of lysozyme without further pretreatment. The MIP-based fluorescent receptor exhibited good selectivity and sensitivity for lysozyme detection. The optimum fluorescence intensity of the MIP-QDs was found to be at pH 6.0, and the linear range of lysozyme was from 0.1 to 2.0 μM with the detection limit of 25.2 nM. Moreover, the proposed fluorescent receptor was satisfactorily applied to the determination of lysozyme in real samples. This study provides a new approach for recognizing and detecting of specific proteins in biological samples.
Vorheriger Artikel The influence of slightly and highly soluble carbonate salts on phase relations in hydrated calcium aluminate cements
Nächster Artikel Controlled synthesis of mesoporous carbons with tailored morphologies via a “silica-assisted” strategy

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Metadaten
Titel
Surface-imprinted polymer coating l-cysteine-capped ZnS quantum dots for target protein specific recognition
verfasst von
Xin Zhang
Shu Yang
Liquan Sun
Aiqin Luo
Publikationsdatum
24.03.2016
Verlag
Springer US
Erschienen in
Journal of Materials Science / Ausgabe 12/2016
Print ISSN: 0022-2461
Elektronische ISSN: 1573-4803
DOI
https://doi.org/10.1007/s10853-016-9914-7

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