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Fluorescence measurement is considered as an effective means of sensing and understanding various molecular processes in chemical and biological science. The time scale of those processes can range from femtoseconds to milliseconds and longer. Longer processes are characterised by changes in physical parameters, often involving temperature (T). Therefore, in addition to time-resolve measurements, T-dependent fluorescence is also important in a variety of applications in sensing. A smartphone intensity fluorimeter capable of measuring time-resolved fluorescence at various temperatures on a smartphone fluorimeter have been demonstrated. The temperature of the sample has been controlled by using a Peltier device powered by smartphone through a microcontroller connected with a temperature sensor, cooling fan and the excitation source. Data sending and receiving are performed from the smartphone app via the Bluetooth communication with the microcontroller. The temperature dependence of four emitters are characterised as a proof-of-principle.
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- Temperature-tunable Smartphone Fluorimeter
Md Arafat Hossain
- Chapter 3
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