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Erschienen in:
1993 | OriginalPaper | Buchkapitel
The Effect of Glutathione Depletion in the Glutamine Synthetase Gene Amplification System
verfasst von : Shue-Yuan Wang, Jane A. Reese, Robert Zivin, Daniel R. Omstead
Erschienen in: Bioproducts and Bioprocesses 2
Verlag: Springer Berlin Heidelberg
Enthalten in: Professional Book Archive
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The glutamine synthetase (GS) system is a novel gene amplification system in which vectors containing GS coding sequences are used as dominant markers amplifiable by selection with methionine sulfoximine (MSX). DNA encoding the desired protein is combined and co-amplified with the GS gene. Transfected cells are cultured in glutamine-free medium with 500 uM MSX to potentiate the amplification.