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Erschienen in:

2015 | OriginalPaper | Buchkapitel

68. The New Strategy of Breeding Cytidine Excessive Biosynthesis Mutants by pyr Operon Rearrangement of Bacillus amyloliquefaciens

verfasst von : Qing Wu, Huiyan Liu, Haitian Fang, Jianguo He, Xiaoguang He, Linan Yu

Erschienen in: Advances in Applied Biotechnology

Verlag: Springer Berlin Heidelberg

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Abstract

Cytidine is a good antitumor and antiviral intermediate which can also be used as a healthy food ingredients. With the market for cytidine increasing, the large-scale production of cytidine by microbial fermentation method has become a major way to solve this problem. While improving cytidine production, the regulation of pyr operon is essential to the excessive synthesis cytidine. However, its transcriptional regulation mechanism is unclear. The passage summarizes the regulation of de novo pyrimidine nucleotide biosynthesis (pyr genes) and the metabolic regulation mechanism of cytidine synthesis in Bacillus amyloliquefaciens. This paper makes the regulation protein pyrR as the research object, presents a series of Bacillus amyloliquefaciens pyrimidine operon transcriptional regulatory rearrangement strategy, effects the transcriptional regulation on cytidine biosynthesis, and aims to provide a theoretical basis for cytidine yielding strain.

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Titel: The New Strategy of Breeding Cytidine Excessive Biosynthesis Mutants by pyr Operon Rearrangement of Bacillus amyloliquefaciens
verfasst von: Qing Wu
Huiyan Liu
Haitian Fang
Jianguo He
Xiaoguang He
Linan Yu
Verlag: Springer Berlin Heidelberg
Buch: Advances in Applied Biotechnology
Print ISBN: 978-3-662-46317-8

Electronic ISBN: 978-3-662-46318-5

Copyright-Jahr: 2015
DOI: https://doi.org/10.1007/978-3-662-46318-5_68

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