Skip to main content
Erschienen in: BioControl 1/2011

01.02.2011

The role of synergistic action and molecular mechanism in the effect of genetically engineered strain Bacillus subtilis OKBHF in enhancing tomato growth and Cucumber mosaic virus resistance

verfasst von: Shuai Wang, Huijun Wu, Jiang Zhan, Yanfei Xia, Shengfeng Gao, Weiduo Wang, Pengqi Xue, Xuewen Gao

Erschienen in: BioControl | Ausgabe 1/2011

Einloggen

Aktivieren Sie unsere intelligente Suche um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

Bacillussubtilis is well known for its biocontrol activity against several plant pathogens and for its role in promoting plant growth. HpaGXooc, from rice pathogenic bacterium Xanthomonasoryzae pv. oryzicola, is a member of the harpin group of proteins. It is known to elicit hypersensitive cell death in non-host plants, thereby inducing disease and insect resistance in the plants and enhancing plant growth. In our previous experiment, we constructed a genetically engineered strain—B. subtilis OKBHF—through the introduction of the gene encoding HpaGXooc into B. subtilis OKB105 in order to combine the effects of HpaGXooc and wild-type PGPR in improving the plant growth rate and for biological control. In this study, we evaluated the use of treating the tomato plant with B. subtilis OKBHF. The results of greenhouse experiments demonstrated that OKBHF treatment had a significant effect on increasing the height, fresh weight, and flower and fruit number and obviously lowered the disease severity of Cucumber mosaic virus (CMV) infection at 28 days postinoculation (dpi). Subsequent reverse transcription-polymerase chain reaction analysis revealed the molecular mechanisms of HpaGXooc and B. subtilis in the tomato plant, suggesting their synergistic roles in inducing enhanced expression of three expansin genes LeEXP2, LeEXP5, and LeEXP18, which regulate plant cell growth, and two defense-related genes Pti4 and Pti6, which activate the expression of a wide array of PR genes and one defense gene, PR-1a.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Literatur
Zurück zum Zitat Asaka O, Shoda M (1996) Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Appl Environ Microbiol 62:4081–4085PubMed Asaka O, Shoda M (1996) Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Appl Environ Microbiol 62:4081–4085PubMed
Zurück zum Zitat Choi D, Lee Y, Cho HT, Kende H (2003) Regulation of expansin gene expression affects growth and development in transgenic rice plants. Plant Cell 15:1386–1398CrossRefPubMed Choi D, Lee Y, Cho HT, Kende H (2003) Regulation of expansin gene expression affects growth and development in transgenic rice plants. Plant Cell 15:1386–1398CrossRefPubMed
Zurück zum Zitat Dobbelaere S, Vanderleyden J, Okon Y (2003) Plant growth-promoting effects of diazotrophs in the rhizosphere. Crit Rev Plant Sci 22:107–149CrossRef Dobbelaere S, Vanderleyden J, Okon Y (2003) Plant growth-promoting effects of diazotrophs in the rhizosphere. Crit Rev Plant Sci 22:107–149CrossRef
Zurück zum Zitat Dong HP, Peng J, Bao Z, Meng X, Bonasera JM, Chen G, Beer SV, Dong H (2004) Downstream divergence of the ethylene signaling pathway for harpin-stimulated Arabidopsis growth and insect defense. Plant Physiol 136:3628–3638CrossRefPubMed Dong HP, Peng J, Bao Z, Meng X, Bonasera JM, Chen G, Beer SV, Dong H (2004) Downstream divergence of the ethylene signaling pathway for harpin-stimulated Arabidopsis growth and insect defense. Plant Physiol 136:3628–3638CrossRefPubMed
Zurück zum Zitat Dong HP, Yu H, Bao Z, Guo X, Peng J, Yao Z, Chen G, Qu S, Dong H (2005) The ABI2-dependent abscisic acid signalling controls HrpN-induced drought tolerance in Arabidopsis. Planta 221:313–327CrossRefPubMed Dong HP, Yu H, Bao Z, Guo X, Peng J, Yao Z, Chen G, Qu S, Dong H (2005) The ABI2-dependent abscisic acid signalling controls HrpN-induced drought tolerance in Arabidopsis. Planta 221:313–327CrossRefPubMed
Zurück zum Zitat Idris EE, Bochow H, Ross H, Borriss R (2004) Use of Bacillus subtilis as biocontrol agent. VI. Phytohormone like action of culture filtrates prepared from plant growth-promoting Bacillus amyloliquefaciens FZB24, FZB42, FZB45 and Bacillus subtilis FZB37. Zeitschrift fuer Pflanzenkrankheiten und Pflanzenschutz 111:583–597 Idris EE, Bochow H, Ross H, Borriss R (2004) Use of Bacillus subtilis as biocontrol agent. VI. Phytohormone like action of culture filtrates prepared from plant growth-promoting Bacillus amyloliquefaciens FZB24, FZB42, FZB45 and Bacillus subtilis FZB37. Zeitschrift fuer Pflanzenkrankheiten und Pflanzenschutz 111:583–597
Zurück zum Zitat Idriss EE, Makarewicz O, Farouk A, Rosner K, Greiner R, Bochow H, Richter T, Borriss R (2002) Extracellular phytase activity of Bacillus amyloliquefaciens FZB45 contributes to its plant-growth-promoting effect. Microbiology 148:2097–2109PubMed Idriss EE, Makarewicz O, Farouk A, Rosner K, Greiner R, Bochow H, Richter T, Borriss R (2002) Extracellular phytase activity of Bacillus amyloliquefaciens FZB45 contributes to its plant-growth-promoting effect. Microbiology 148:2097–2109PubMed
Zurück zum Zitat Kloepper JW, Ryu CM, Zhang S (2004a) Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94:1259–1266CrossRefPubMed Kloepper JW, Ryu CM, Zhang S (2004a) Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94:1259–1266CrossRefPubMed
Zurück zum Zitat Kloepper JW, Reddy MS, Kenney DS, Vavrina C, Kokalis-Burelle N, Martinez-Ochoa N (2004b) Application for rhizobacteria in transplant production and yield enhancement. In: Nicola S, Nowak J, Vavrina CS (eds) XXVI International horticultural congress: issues and advances in transplant production and stand establishment research, Toronto, Canada, Acta Hort, vol 631, pp 219–229 Kloepper JW, Reddy MS, Kenney DS, Vavrina C, Kokalis-Burelle N, Martinez-Ochoa N (2004b) Application for rhizobacteria in transplant production and yield enhancement. In: Nicola S, Nowak J, Vavrina CS (eds) XXVI International horticultural congress: issues and advances in transplant production and stand establishment research, Toronto, Canada, Acta Hort, vol 631, pp 219–229
Zurück zum Zitat Krause MS, De Ceuster TJ, Tiquia SM, Michel FC, Madden LV, Hoitink HA (2003) Isolation and characterization of rhizobacteria from composts that suppress the severity of bacterial leaf spot of radish. Phytopathology 93:1292–1300CrossRefPubMed Krause MS, De Ceuster TJ, Tiquia SM, Michel FC, Madden LV, Hoitink HA (2003) Isolation and characterization of rhizobacteria from composts that suppress the severity of bacterial leaf spot of radish. Phytopathology 93:1292–1300CrossRefPubMed
Zurück zum Zitat Landy M, Warren GH, Rosenman SB, Colio LG (1948) Bacillomycin; an antibiotic from Bacillus subtilis active against pathogenic fungi. Proc Soc Exp Biol Med 67:539–541PubMed Landy M, Warren GH, Rosenman SB, Colio LG (1948) Bacillomycin; an antibiotic from Bacillus subtilis active against pathogenic fungi. Proc Soc Exp Biol Med 67:539–541PubMed
Zurück zum Zitat Ma LL, Huo R, Gao XW, He D, Shao M, Wang Q (2008) Transgenic rape with hrf2 gene encoding harpinXooc resistant to Sclerotinia sclerotinorium. J Agric Sci China 7:455–461 Ma LL, Huo R, Gao XW, He D, Shao M, Wang Q (2008) Transgenic rape with hrf2 gene encoding harpinXooc resistant to Sclerotinia sclerotinorium. J Agric Sci China 7:455–461
Zurück zum Zitat Nandakumar R, Babu S, Viswanathan R, Raguchander T, Samiyappan R (2001) Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens. J Soil Biol Biochem 33:603–612CrossRef Nandakumar R, Babu S, Viswanathan R, Raguchander T, Samiyappan R (2001) Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens. J Soil Biol Biochem 33:603–612CrossRef
Zurück zum Zitat Ongena M, Duby F, Jourdan E, Beaudry T, Jadin V, Dommes J, Thonart P (2005) Bacillus subtilis M4 decreases plant susceptibility towards fungal pathogens by increasing host resistance associated with differential gene expression. Appl Microbiol Biotechnol 67:692–698CrossRefPubMed Ongena M, Duby F, Jourdan E, Beaudry T, Jadin V, Dommes J, Thonart P (2005) Bacillus subtilis M4 decreases plant susceptibility towards fungal pathogens by increasing host resistance associated with differential gene expression. Appl Microbiol Biotechnol 67:692–698CrossRefPubMed
Zurück zum Zitat Peng JL, Dong HS, Dong HP, Delaney TP, Bonasera JM, Beer SV (2003) Harpin-elicited hypersensitive cell death and pathogen resistance require the NDR1 and EDS1 genes. Physiol Mol Plant Pathol 62:317–326CrossRef Peng JL, Dong HS, Dong HP, Delaney TP, Bonasera JM, Beer SV (2003) Harpin-elicited hypersensitive cell death and pathogen resistance require the NDR1 and EDS1 genes. Physiol Mol Plant Pathol 62:317–326CrossRef
Zurück zum Zitat Ryu CM, Farag MA, Hu CH, Reddy MS, Wei HX, Pare PW, Kloepper JW (2003) Bacterial volatiles promote growth in Arabidopsis. Proc Natl Acad Sci USA 100:4927–4932CrossRefPubMed Ryu CM, Farag MA, Hu CH, Reddy MS, Wei HX, Pare PW, Kloepper JW (2003) Bacterial volatiles promote growth in Arabidopsis. Proc Natl Acad Sci USA 100:4927–4932CrossRefPubMed
Zurück zum Zitat Ryu CM, Farag MA, Hu CH, Reddy MS, Kloepper JW, Pare PW (2004) Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiol 134:1017–1026CrossRefPubMed Ryu CM, Farag MA, Hu CH, Reddy MS, Kloepper JW, Pare PW (2004) Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiol 134:1017–1026CrossRefPubMed
Zurück zum Zitat Saravanakumar D, Vijayakumar C, Kumar N, Samiyappan R (2007) PGPR-induced defense responses in the tea plant against blister blight disease. Crop Prot 26:556–565CrossRef Saravanakumar D, Vijayakumar C, Kumar N, Samiyappan R (2007) PGPR-induced defense responses in the tea plant against blister blight disease. Crop Prot 26:556–565CrossRef
Zurück zum Zitat Tjamos SE, Flemetakis E, Paplomatas EJ, Katinakis P (2005) Induction of resistance to Verticillium dahliae in Arabidopsis thaliana by the biocontrol agent K-165 and pathogenesis-related proteins gene expression. Mol Plant Microbe Interact 18:555–561CrossRefPubMed Tjamos SE, Flemetakis E, Paplomatas EJ, Katinakis P (2005) Induction of resistance to Verticillium dahliae in Arabidopsis thaliana by the biocontrol agent K-165 and pathogenesis-related proteins gene expression. Mol Plant Microbe Interact 18:555–561CrossRefPubMed
Zurück zum Zitat Touré Y, Ongena M, Jacques P, Guiro A, Thonart P (2004) Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould disease caused by Botrytis cinerea on apple. J Appl Microbiol 96:1151–1160CrossRefPubMed Touré Y, Ongena M, Jacques P, Guiro A, Thonart P (2004) Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould disease caused by Botrytis cinerea on apple. J Appl Microbiol 96:1151–1160CrossRefPubMed
Zurück zum Zitat van Loon LC, Bakker PA, Pieterse CM (1998) Systemic resistance induced by rhizosphere bacteria. Annu Rev Phytopathol 36:453–483CrossRefPubMed van Loon LC, Bakker PA, Pieterse CM (1998) Systemic resistance induced by rhizosphere bacteria. Annu Rev Phytopathol 36:453–483CrossRefPubMed
Zurück zum Zitat Vollenbroich D, Pauli G, Ozel M, Vater J (1997) Antimycoplasma properties and application in cell culture of surfactin, a lipopeptide antibiotic from Bacillus subtilis. Appl Environ Microbiol 63:44–49PubMed Vollenbroich D, Pauli G, Ozel M, Vater J (1997) Antimycoplasma properties and application in cell culture of surfactin, a lipopeptide antibiotic from Bacillus subtilis. Appl Environ Microbiol 63:44–49PubMed
Zurück zum Zitat Wang S, Wu HJ, Qiao JQ, Ma LL, Liu J, Xia YF, Gao XW (2009) Molecular mechanism of plant growth promotion and induced systemic resistance to tobacco mosaic virus by Bacillus spp. J Microbiol Biotechnol 19:1250–1258CrossRefPubMed Wang S, Wu HJ, Qiao JQ, Ma LL, Liu J, Xia YF, Gao XW (2009) Molecular mechanism of plant growth promotion and induced systemic resistance to tobacco mosaic virus by Bacillus spp. J Microbiol Biotechnol 19:1250–1258CrossRefPubMed
Zurück zum Zitat Wu K, Tian L, Hollingworth J, Brown DC, Miki B (2002) Functional analysis of tomato Pti4 in Arabidopsis. Plant Physiol 128:30–37CrossRefPubMed Wu K, Tian L, Hollingworth J, Brown DC, Miki B (2002) Functional analysis of tomato Pti4 in Arabidopsis. Plant Physiol 128:30–37CrossRefPubMed
Zurück zum Zitat Wu HJ, Wang S, Qiao JQ, Liu J, Zhan J, Gao XW (2009) Expression of HpaGXooc protein in Bacillus subtilis and its biological functions. J Microbiol Biotechnol 19:194–203CrossRefPubMed Wu HJ, Wang S, Qiao JQ, Liu J, Zhan J, Gao XW (2009) Expression of HpaGXooc protein in Bacillus subtilis and its biological functions. J Microbiol Biotechnol 19:194–203CrossRefPubMed
Metadaten
Titel
The role of synergistic action and molecular mechanism in the effect of genetically engineered strain Bacillus subtilis OKBHF in enhancing tomato growth and Cucumber mosaic virus resistance
verfasst von
Shuai Wang
Huijun Wu
Jiang Zhan
Yanfei Xia
Shengfeng Gao
Weiduo Wang
Pengqi Xue
Xuewen Gao
Publikationsdatum
01.02.2011
Verlag
Springer Netherlands
Erschienen in
BioControl / Ausgabe 1/2011
Print ISSN: 1386-6141
Elektronische ISSN: 1573-8248
DOI
https://doi.org/10.1007/s10526-010-9306-x

Weitere Artikel der Ausgabe 1/2011

BioControl 1/2011 Zur Ausgabe