We report about the investigation of the amoeboid locomotion at
. Based on the detailed experimental study of the internal cytoplasm flow and the variation of the contour of the amoeba with optical flow measurement techniques like particle image velocimetry (PIV) we found characteristic velocity fields and motions of the center of mass. Furthermore a peripheral cell model is developed, in which a contractile backward flow of actin-myosin in the cortex stabilizes cell polarity and locomotion by inducing more protrusions in the front and stronger retraction in the rear. The results from the experimental and theoretical study were used to realise prototypes of locomotion systems, composed of silicon elastomer body with controlled elasticity and driven by a magnetic system, based on amoeboid motion principles.