One of the important roles of cytoskeleton is to maintain global mechanical strength of the cell. It is expected the effect of environmental mechanical stimulations appears in cell morphology. An environment of cultured cells in an ordinary incubator might be inappropriate, because those are normally free from mechanical stimulations such as fluid flow shear stress, tensile strain, compression and vibration. In this study, a three-dimensional micro vibration stage, to exert vibration stimulation non-invasively and three-dimensionally onto cultured cells, is developed. This vibration stage is assumed to be installed and operated in a CO
incubator, so it has a compact and simple cantilever structure. In order to excite vibrations in each direction of orthogonal coordinate system, it has made from one stainless steel strip into a V-shaped vibrator, which is kinetically designed. Three piezoelectric ceramics were bonded on the vibrator, so that it is able to apply the three-dimensional vibration stimulation onto the cells on the culture dish. By using the developed stage, the effect of vibration stimulation was investigated morphologically. The experimental result shows that the vibration stimulation decreases the projected area and increases the slenderness ratio. In other words, the dynamic stimulation inhibits the progression of morphological change according to cell senescence. This might suggest that the stimulation affects gene expression pattern. Also, the most effective condition of vibration was horizontal direction of 10 Hz. It is found that the developed micro vibration stage is quite useful as a control device for cell culture.