2014 | OriginalPaper | Buchkapitel
Towards Point-of-Use Dielectrophoretic Methods: A New Portable Multiphase Generator for Bacteria Concentration
verfasst von : B. del Moral Zamora, J. M. Álvarez Azpeitia, J. Colomer Farrarons, P. Ll. Miribel Català, A. Homs Corbera, A. Juárez, J. Samitier
Erschienen in: XIII Mediterranean Conference on Medical and Biological Engineering and Computing 2013
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This manuscript presents portable and low cost electronic system for specific point-of-use dielectrophoresis applications. The system is composed of two main modules: a) a multiphase generator based on a Class E amplifier, which provides 4 sinusoidal signals (0º, 90º, 180º, 270º) at 1 MHz with variable output voltage up to 10 Vpp (Vm) and an output driving current of 1 A; and b) a dielectrophoresis-based microfluidic chip containing two interdigitated electrodes. The system has been validated by concentrating Escherichia Coli at 1 MHz while applying a continuous flow of 5
μ
L/min. Device functionalities were verified under different conditions achieving a 83% trapping efficiency in the best case.