nach oben

Biomass Conversion and Biorefinery

Erschienen in:

11.02.2020 | Original Article

Ultrasound-assisted extraction ameliorates the physicochemical properties of defatted mulberry seed protein to promote lipid production in Schizochytrium sp. SR21

verfasst von: Wen-Jing Li, Yu-Rong Bian, Ya-Li Chai, Hang-Xing Ding, Sheng Sheng, Fu-An Wu, Jun Wang

Erschienen in: Biomass Conversion and Biorefinery | Ausgabe 2/2021

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config

KI-gestützte Suche

Aus

Abstract

To improve protein extraction rate and the utilization of mulberry seed protein (MSP) for biorefinery purposes, ultrasound-assisted extraction was used to treating defatted mulberry seed protein (DMSP) for enhancing lipid production in Schizochytrium sp. SR21. Under ultrasound irradiation with solid-liquid ratio of 1:40, temperature of 60 °C, time of 9 min, and output power of 600 W, the extraction rate of DMSP reached 77.05%, which was significantly improved than the blank control (18.70%) and microwave treatment (22.50%). Moreover, the solubility and emulsibility of DMSP treated by ultrasound were significantly improved; the assay results measured by near-infrared Raman Spectroscopy showed that ultrasound-assisted treatment could reduce the α-helix in the protein (reduced by 9.75%); correspondingly, structures of β-sheet and β-turn increased 1.33% and 3.01%; it indicated that ultrasound treatment changed the spatial arrangement of protein main chain atoms. The antioxidant activity test and the amino acid composition of defatted mulberry seed protein hydrolysate (DMSPH) showed that ultrasound-assisted treatment enhanced the antioxidant activity of the protein hydrolysate and there was no significant difference in amino acid composition between the DMSP and yeast extract. The DMSPH was used as nitrogen source to cultivate Schizochytrium sp. SR21 which showed that DMSPH promoted lipid production and the DHA content was 35.55%, which was better than the results of cultured with traditional nitrogen source; it indicates that DMSP can be used as nitrogen source in culture of microorganisms and further explanation of ultrasound-assisted treatment of modified MSP as an alternative nitrogen source achieved the goal of biorefinery.

Vorheriger Artikel Thermochemical conversion of apple seeds before and after supercritical CO2 extraction: an assessment through evolved gas analysis

Nächster Artikel β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Nur mit Berechtigung zugänglich

Joglekar SN, Pathak PD, Mandavgane SA, Kulkarni BD (2019) Process of fruit peel waste biorefinery: a case study of citrus waste biorefinery, its environmental impacts and recommendations. Environ Sci Pollut Res 26:34713–34722. https://doi.org/10.1007/s11356-019-04196-0CrossRef

Meinita MDN, Marhaeni B, Jeong GT, Hong YK (2019) Sequential acid and enzymatic hydrolysis of carrageenan solid waste for bioethanol production: a biorefinery approach. J Appl Phycol 31(4):2507–2515. https://doi.org/10.1007/s10811-019-1755-8CrossRef

Corona A, Biddy MJ, Vardon DR et al (2018) Life cycle assessment of adipic acid production from lignin. Green Chem 20:3857–3866. https://doi.org/10.1039/C8GC00868JCrossRef

Ditta YA, King AJ (2017) Recent advances in sunflower seed meal as an alternate source of protein in broilers. Worlds Poultry Sci J 73(03):527–542. https://doi.org/10.1017/S0043933917000423CrossRef

Kang H, Wang Z, Wang Y et al (2019) Development of mainly plant protein-derived plywood bioadhesives via soy protein isolate fiber self-reinforced soybean meal composites. Ind Crop Prod 133:10–17. https://doi.org/10.1016/j.indcrop.2019.03.022CrossRef

Ugolini L, Cinti S, Righetti L et al (2015) Production of an enzymatic protein hydrolyzate from defatted sunflower seed meal for potential application as a plant biostimulant[J]. Ind Crop Prod 75:15–23. https://doi.org/10.1016/j.indcrop.2014.11.026CrossRef

Chang J, Kang X, Yuan JL (2018) Enhancing emulsification and antioxidant ability of egg albumin by moderately acid hydrolysis: modulating an emulsion-based system for mulberry seed oil. Food Res Int 109:334–342. https://doi.org/10.1016/j.foodres.2018.04.055CrossRef

Oh M, Bae SY, Chung MS (2013) Mulberry (Morus alba) seed extract and its polyphenol compounds for control of foodborne viral surrogates. J Korean Soc Appl Biol Chem 56(6):655–660. https://doi.org/10.1007/s13765-013-3266-7CrossRef

Zhang L, Zheng P, Shen K et al (2018) Influence of ultrasound-assisted alkali treatment on the structural properties and functionalities of rice protein. J Cereal Sci 79:204–209. https://doi.org/10.1016/j.jcs.2017.10.013CrossRef

10.

Yao Z, Vanga SK, Jin W et al (2018) Effects of utrasonic and microwave processing on avidin assay and secondary structures of egg white protein. Food Bioprocess Technol 11:1974–1984. https://doi.org/10.1007/s11947-018-2158-6CrossRef

11.

Uluko H, Zhang S, Lu L et al (2015) Effects of thermal, microwave, and ultrasound pretreatments on antioxidative capacity of enzymatic milk protein concentrate hydrolysates. J Funct Foods 18:1138–1146. https://doi.org/10.1016/j.jff.2014.11.024CrossRef

12.

Borah PP, Das P, Badwaik LS (2017) Ultrasound treated potato peel and sweet lime pomace based biopolymer film development. Ultrason Sonochem 36:11–19. https://doi.org/10.1016/j.ultsonch.2016.11.010CrossRef

13.

Xu XH, Liu ZX, Shi XY, Miao C, Sheng S, Xu Y, Wu FA, Wang J (2018) Fed-batch fermentation of Yarrowia lipolytica using defatted silkworm pupae hydrolysate: A dynamic model-based approach for high yield of lipid production. Waste Biomass Valorization 12:2399–2411. https://doi.org/10.1007/s12649-017-0180-yCrossRef

14.

Liu ZX, You S, Tang BP, Wang B, Sheng S, Wu FA, Wang J (2019) Inositol as a new enhancer for improving lipid production and accumulation in Schizochytrium sp. SR21. Environ Sci Pollut Res 26:35497–35508. https://doi.org/10.1007/s11356-019-06056-3CrossRef

15.

Cunshan Z, Haile M, Xiaojie Y et al (2013) Pretreatment of defatted wheat germ proteins (by-products of flour mill industry) using ultrasonic horn and bath reactors: effect on structure and preparation of ACE-inhibitory peptides. Ultrason Sonochem 20(6):1390–1400. https://doi.org/10.1016/j.ultsonch.2013.04.005CrossRef

16.

Keskin S, Yalçin E, H Ö. (2018) Effects of storage and Granary weevil infestation on gel electrophoresis and protein solubility properties of hard and soft wheat flours. J Econ Entomol 111(3):1454–1460. https://doi.org/10.1093/jee/toy041CrossRef

17.

Jiang J, Yan J, Liang X, Piatko M et al (2018) Synergetic interfacial adsorption of protein and low-molecular-weight emulsifiers in aerated emulsions. Food Hydrocoll 81:15–22CrossRef

18.

Zhang L, Wang LJ, Jiang W, Qian JY (2017) Effect of pulsed electric field on functional and structural properties of canola protein by pretreating seeds to elevate oil yield. LWT Food Sci Technol 84:73–81CrossRef

19.

Zabielski J, Kijowski J, Fiszer W, Niewiarowicz A (2010) The effect of irradiation on technological properties and protein solubility of broiler chicken meat. J Sci Food Agric 35:662–670CrossRef

20.

Russo L, Sánchez-Purrà M, Rodriguez-Quijada C, Leonardo BM, Puntes V, Hamad-Schifferli K (2019) Detection of resistance protein A (MxA) in paper-based immunoassays with surface enhanced Raman spectroscopy with AuAg nanoshells. Nanoscale 11:10819–10827. https://doi.org/10.1039/C9NR02397FCrossRef

21.

Fujiya NM, Tavares MFM (2003) Analysis of underivatized amino acids in protein hydrolysates for cosmetic application by capillary electrophoresis. J Sep Sci 26(6–7):562–568. https://doi.org/10.1002/jssc.200390077CrossRef

22.

Musa KH, Abdullah A, Al-Haiqi A (2016) Determination of DPPH free radical scavenging activity: application of artificial neural networks. Food Chem 194(4):705–711. https://doi.org/10.1016/j.foodchem.2015.08.038CrossRef

23.

Dabbour M, He R, Mintah B et al (2019) Changes in functionalities, conformational characteristics and antioxidative capacities of sunflower protein by controlled enzymolysis and ultrasonication action. Ultrason Sonochem 104625. https://doi.org/10.1016/j.ultsonch.2019.104625

24.

Patil KP, Gogate PR (2015) Improved synthesis of docosahexaenoic acid (DHA) using Schizochytrium limacinum SR21 and sustainable media. Chem Eng J 268:187–196. https://doi.org/10.1016/j.cej.2015.01.050CrossRef

25.

Wang J, Liu X, Wang XD, Dong T, Zhao XY, Zhu D, Mei YY, Wu GH (2016) Selective synthesis of human milk fat-style structured triglycerides from microalgal oil in a microfluidic reactor packed with immobilized lipase. Bioresour Technol 220:132–141. https://doi.org/10.1016/j.biortech.2016.08.023CrossRef

26.

Zhao XY, Wang XD, Liu X et al (2015) Structured lipids enriched with unsaturated fatty acids produced by enzymatic acidolysis of silkworm pupae oil using oleic acid. Eur J Lipid Sci Technol 117(6):879–889. https://doi.org/10.1002/ejlt.201400438CrossRef

27.

Lee JH, Son JM, Akoh CC, Kim MR, Lee KT (2010) Optimized synthesis of 1, 3-dioleoyl-2-palmitoylglycerol-rich triacylglycerol via interesterification catalyzed by a lipase from Thermomyces lanuginosus. New Biotechnol 27(1):38–45. https://doi.org/10.1016/j.nbt.2009.10.006CrossRef

28.

Ramin T, Mohammad-Sadegh H, Morteza K et al (2019) Ultrasound-assisted packed-bed extraction of hypericin from Hypericum perforatum L. and optimization by response surface methodology. Ultrason Sonochem 57:89–97. https://doi.org/10.1016/j.ultsonch.2019.05.018CrossRef

29.

Xavier L, Freire MS, González-álvarez J (2019) Modeling and optimizing the solid–liquid extraction of phenolic compounds from lignocellulosic subproducts. Biomass Conversion Biorefinery 9:737–747. https://doi.org/10.1007/s13399-019-00401-9CrossRef

30.

Manasa P, Saroj P, Korrapati N (2018) Ultrasound-assisted alkaline pretreatment to intensify enzymatic saccharification of Crotalaria juncea using a statistical method. Biomass Conversion Biorefinery 8(3):659–668. https://doi.org/10.1007/s13399-018-0324-8CrossRef

31.

Villa-Vélez HA, Cornelio ML, Corrêa JLG, Telis-Romero J (2017) Identification of acoustic fields in aqueous biomass solutions of banana waste pretreated by power ultrasound. Biomass Conversion Biorefinery 8(1):87–96. https://doi.org/10.1007/s13399-017-0256-8CrossRef

32.

Yang X, Li Y, Li S, Oladejo AO, Wang Y, Huang S, Zhou C, Wang Y, Mao L, Zhang Y, Ma H, Ye X (2017) Effects of low power density muti-frequency ultrasound pretreatment on the enzymolysis and the structure characterization of defatted wheat germ protein. Ultrason Sonochem 38:410–420. https://doi.org/10.1016/j.ultsonch.2017.03.001CrossRef

33.

Xing F, Qiao L, Chao QT et al (2019) Study on structural, rheological and foaming properties of ovalbumin by ultrasound-assisted glycation with xylose. Ultrason Sonochem 58:104644. https://doi.org/10.1016/j.ultsonch.2019.104644CrossRef

34.

Oboroceanu D, Wang L, Magner E et al (2014) Fibrillization of whey proteins improves foaming capacity and foam stability at low protein concentrations. J Food Eng 121(1):102–111. https://doi.org/10.1016/j.jfoodeng.2013.08.023CrossRef

35.

Xiong T, Xiong W, Ge M, Xia J, Li B, Chen Y (2018) Effect of high intensity ultrasound on structure and foaming properties of pea protein isolate. Food Res Int 109:260–267. https://doi.org/10.1016/j.foodres.2018.04.044CrossRef

36.

Guimarães JT, Balthazar CF, Scudino H, Pimentel TC, Esmerino EA, Ashokkumar M, Freitas MQ, Cruz AG (2019) High-intensity ultrasound: A novel technology for the development of probiotic and prebiotic dairy products. Ultrason Sonochem 57:12–21. https://doi.org/10.1016/j.ultsonch.2019.05.004CrossRef

37.

Panaras G, Moatsou G, Yanniotis S et al (2011) The influence of functional properties of different whey protein concentrates on the rheological and emulsification capacity of blends with xanthan gum. Carbohydr Polym 86(2):433–440. https://doi.org/10.1016/j.carbpol.2011.03.044CrossRef

38.

Hadnađev M, Dapčević-Hadnađev T, Lazaridou A et al (2018) Hempseed meal protein isolates prepared by different isolation techniques. Part I. physicochemical properties. Food Hydrocoll 79:526–533. https://doi.org/10.1016/j.foodhyd.2017.12.015CrossRef

39.

Vera A, Valenzuela MA, Yazdani-Pedram M, Tapia C, Abugoch L (2019) Conformational and physicochemical properties of quinoa proteins affected by different conditions of high-intensity ultrasound treatments. Ultrason Sonochem 51:186–196. https://doi.org/10.1016/j.ultsonch.2018.10.026CrossRef

40.

Stanislavov AS, Sukhodub LF, Sukhodub LB, Kuznetsov VN, Bychkov KL, Kravchenko MI (2018) Structural features of hydroxyapatite and carbonated apatite formed under the influence of ultrasound and microwave radiation and their effect on the bioactivity of the nanomaterials. Ultrason Sonochem 42:84–96. https://doi.org/10.1016/j.ultsonch.2017.11.011CrossRef

41.

Mouritsen OG, Duelund L, Petersen MA et al (2019) Umami taste, free amino acid composition, and volatile compounds of brown seaweeds. J Appl Phycol 31(2):1213–1232. https://doi.org/10.1007/s13399-017-0256-8CrossRef

42.

Mutalikdesai A, Nassir M, Saady A, Hassner A, Gedanken A (2019) Sonochemically modified ovalbumin enhances Enantioenrichment of some amino acids. Ultrason Sonochem 58:104603. https://doi.org/10.1016/j.ultsonch.2019.05.020CrossRef

43.

Mokhtar D, Ronghai H, Benjamin M et al (2019) Changes in functionalities, conformational characteristics and antioxidative capacities of sunflower protein by controlled enzymolysis and ultrasonication action. Ultrason Sonochem 58:104625. https://doi.org/10.1016/j.ultsonch.2019.104625CrossRef

44.

Xu C, Qiao L, Guo Y, Ma L, Cheng Y (2018) Preparation, characteristics and antioxidant activity of polysaccharides and proteins-capped selenium nanoparticles synthesized by Lactobacillus casei 393. Carbohydr Polym 195:576–585. https://doi.org/10.1016/j.carbpol.2018.04.110CrossRef

45.

Han X, Shen K, Huang G et al (2018) Synthesis, structure, electrochemical properties and superoxide radical scavenging activities of two thiocyanate copper (II) complexes with different pyridyl-benzoxazole ligands. J Mol Struct 1169:18–24. https://doi.org/10.1016/j.molstruc.2018.05.058CrossRef

46.

Ai M, Tang T, Zhou L et al (2019) Effects of different proteases on the emulsifying capacity, rheological and structure characteristics of preserved egg white hydrolysates. Food Hydrocoll 87:933–942. https://doi.org/10.1016/j.foodhyd.2018.09.023CrossRef

Titel: Ultrasound-assisted extraction ameliorates the physicochemical properties of defatted mulberry seed protein to promote lipid production in Schizochytrium sp. SR21
verfasst von: Wen-Jing Li
Yu-Rong Bian
Ya-Li Chai
Hang-Xing Ding
Sheng Sheng
Fu-An Wu
Jun Wang
Publikationsdatum: 11.02.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: Biomass Conversion and Biorefinery / Ausgabe 2/2021
Print ISSN: 2190-6815
Elektronische ISSN: 2190-6823
DOI: https://doi.org/10.1007/s13399-020-00626-z

Springer Professional

Abstract

Bitte loggen Sie sich ein, um Zugang zu Ihrer Lizenz zu erhalten.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Weitere Artikel der Ausgabe 2/2021

Simulation and optimization of combined acid pretreatment and enzymatic saccharification of medical cotton waste

Individual torrefaction parameter enhances characteristics of torrefied empty fruit bunches

Valorisation of restaurant food waste under the concept of a biorefinery

Biochars obtained from freshwater biomass—green macroalga and hornwort as Cr(III) ions sorbents

Biochar production from sewage sludge and microalgae mixtures: properties, sustainability and possible role in circular economy

Flame propagation and burning characteristics of pulverized biomass for sustainable biofuel