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Erschienen in: Journal of Nanoparticle Research 10/2011

01.10.2011 | Research Paper

Using fluorescence measurement of zinc ions liberated from ZnS nanoparticle labels in bioassay for Escherichia coli O157:H7

verfasst von: Chad L. Cowles, Xiaoshan Zhu, Chi-Yun Pai

Erschienen in: Journal of Nanoparticle Research | Ausgabe 10/2011

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Abstract

In this study, an alternative approach using ZnS nanoparticle biolabels as fluorescence signal transducers is reported for the immunoassay of E. coli O157:H7 in tap water samples. Instead of measuring the fluorescence of ZnS nanoparticles in the assay, the fluorescence signal is generated through the binding of zinc ions released from nanoparticle labels with zinc-ion sensitive fluorescence indicator Fluozin-3. In the assay, ZnS nanoparticles around 50 nm in diameter were synthesized, bioconjugated, and applied for the detection of E. coli O157:H7. The assay shows a detection range over two orders of magnitude and a detection limit around 1000 colony-forming units (cfu) of E. coli O157:H7.

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Metadaten
Titel
Using fluorescence measurement of zinc ions liberated from ZnS nanoparticle labels in bioassay for Escherichia coli O157:H7
verfasst von
Chad L. Cowles
Xiaoshan Zhu
Chi-Yun Pai
Publikationsdatum
01.10.2011
Verlag
Springer Netherlands
Erschienen in
Journal of Nanoparticle Research / Ausgabe 10/2011
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-011-0528-3

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