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Erschienen in:

2015 | Online First | Buchkapitel

Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts into Induced Pluripotent Stem Cells (iPSCs)

verfasst von : Lucas V. Greder, Jason Post, James R. Dutton

Erschienen in: Methods in Molecular Biology™

Verlag: Springer New York

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Abstract

The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) using a combination of defined transcription factors has become one of the most widely used techniques in stem cell biology. A critical, early event in iPSC reprogramming is the induction of the endogenous transcription factor network that maintains pluripotency in iPSCs. Here we describe using a transgenic, conditional Oct4-Cre construct to investigate the spatial and temporal induction of endogenous Oct4 expression during the reprogramming of mouse fibroblasts into iPS cells.

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Titel: Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts into Induced Pluripotent Stem Cells (iPSCs)
verfasst von: Lucas V. Greder
Jason Post
James R. Dutton
Verlag: Springer New York
DOI: https://doi.org/10.1007/7651_2015_198

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Abstract

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