Skip to main content
Fachgebiete
Automobil + Motoren Bauwesen + Immobilien Business IT + Informatik Elektrotechnik + Elektronik Energie + Nachhaltigkeit Finance + Banking Management + Führung Marketing + Vertrieb Maschinenbau + Werkstoffe Versicherung + Risiko
DE
EN
Bücher
Zeitschriften
Themenseiten
Organisationspsychologie Projektmanagement Marketing Smart Manufacturing
Weitere Formate
Podcasts Webinare Technik Webinare Wirtschaft Kongresse Veranstaltungskalender Awards
Jetzt Einzelzugang starten
Zugang für Unternehmen
Referenzkunden
SLX-Digitalkonferenz: Zukunftswerkstatt 2024

Mehr Umsatz mit Top-Kontakten

Am 7. Mai erfahren Sie, wie Vertriebsteams Leadgenerierung, Leadnurturing und Leadstrategien effizient steuern können.
Erweiterte Suche
Anmelden
nach oben
Erschienen in:
A Preliminary Assessment of Three Strategies for the Agent-Based Modeling of Bacterial Conjugation Kapitel lesen Erstes Kapitel lesen

2015 | OriginalPaper | Buchkapitel

UV-Visible Spectrophotometry-Based Metabolomic Analysis of Cedrela Fissilis Velozzo (Meliaceae) Calluses - A Screening Tool for Culture Medium Composition and Cell Metabolic Profiles

verfasst von : Fernanda Kokowicz Pilatti, Christopher Costa, Miguel Rocha, Marcelo Maraschin, Ana Maria Viana

Erschienen in: 9th International Conference on Practical Applications of Computational Biology and Bioinformatics

Verlag: Springer International Publishing

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

In plant cell cultures aiming at the production of secondary metabolites of industrial interest, the culture medium composition is a decisive step for obtaining cell growth and high yields of the target compound(s). A rapid and reliable methodology for screening metabolic responses to medium composition is fundamental for the development of this biotechnological field. Following this approach, UV-Vis scanning spectrophotometry of callus extracts and their spectra pre-processing, univariate and multivariate analysis were tested in the present work. The results obtained successfully discriminated the culture media investigated and shed light on what metabolic pathways might be responsible for the differences among the callus cultures’ metabolic profiles.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Wirtschaft"

Online-Abonnement

Mit Springer Professional "Wirtschaft" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 340 Zeitschriften

aus folgenden Fachgebieten:

  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Versicherung + Risiko




Jetzt Wissensvorsprung sichern!

Jetzt informieren
Vorheriges Kapitel UV-Visible Scanning Spectrophotometry and Chemometric Analysis as Tools to Build Descriptive and Classification Models for Propolis from Southern Brazil
Nächstes Kapitel An Integrated Computational Platform for Metabolomics Data Analysis
Literatur
1.
Goulart, H.R., Kimura, E.A., Peres, V.J., Couto, A.S., Aquino Duarte, F.A., Katzin, A.M.: Terpenes arrest parasite development and inhibit biosynthesis of isoprenoids in Plasmodium falciparum. Antimicrob. Agents Chemother. 48, 2502–2509 (2004)CrossRef
2.
Leite, A.C., Bueno, F.C., Oliveira, C.G., Fernandes, J.B., Da Vieira, P.C., Silva, M.F.G.F., Bueno, O.C., Pagnocca, F.C., Hebling, M.J.A., Bacci Jr, M.: Limonoids from Cipadessa fruticosa and Cedrela fissilis and their insecticidal activity. J. Braz. Chem. Soc. 16, 1391–1395 (2005)CrossRef
3.
Ambrozin, A.R.P., Leite, A.C., Bueno, F.C.: Limonoids from andiroba oil and Cedrela fissilis and their insecticidal activity. J. Braz. Chem. Soc. 3, 542–547 (2006)
4.
Ramos, D.F., Leitão, G.G., Costa, F.N., Abreu, L., Villarreal, J.V., Leitão, S.G., Fernández, S.L.S., Da Silva, P.E.A.: Investigation of the antimycobacterial activity of 36 plant extracts from the Brazilian Atlantic Forest. Braz. J. Pharm. Sci. 44, 669–674 (2008)
5.
Leite, A.C., Ambrozin, A.R.P., Castilho, M.S., Vieira, P.C., Fernandes, J.B., Oliva, G., Silva, M.F.G.F., Thiemann, O.H., Lima, M.I., Pirani, J.R.: Screening of Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase enzyme inhibitors. Rev. Bras. Farmacogn. 19, 1–6 (2009)
6.
Nunes, E.C., Castilho, C.V., Moreno, F.N., Viana, A.M.: In vitro culture of Cedrela fissilis Vellozo (Meliaceae). Plant Cell Tiss. Org. 70, 259–268 (2002)CrossRef
7.
Nunes, E.C., Benson, E.E., Oltramari, A.C., Araujo, P.S., Moser, J.R., Viana, A.M.: In vitro conservation of Cedrela fissilis Vellozo (Meliaceae), a native tree of the Brazilian Atlantic Forest. Biodivers. Conserv. 12, 837–848 (2003)CrossRef
8.
Nunes, E.C., Laudano, W.L.S., Moreno, F.N., Castilho, C.V., Mioto, P., Sampaio, F.L., Bortoluzi, J.H., Benson, E.E., Pizolatti, M.G., Carasek, E., Viana, A.M.: Micropropagation of Cedrela fissilis Vell. (Meliaceae). In: Jain, S.M., Häggman, H. (ed.) – Protocols for Micropropagation of Woody Trees and Fruits, pp. 221–235. Springer, The Netherlands (2007)
9.
Nielsen, J., Jewett, M.C.: Metabolomics - A powerful pool in systems biology. Springer-Verlag, Berlin, Heidelberg (2007)
10.
Villas-Bôas, S.G., Roessner, U., Hansen, M.A.E., Smedsgaard, J., Nielsen, J.: Metabolome analysis: An introduction. Wiley, New Jersey (2007)CrossRef
11.
Putri, S.P., Nakayama, Y., Matsuda, F., Uchikata, T., Kobayashi, S., Matsubara, A., Fukusaki, E.: Current metabolomics: practical applications. J. Biosci. Bioeng. 115, 579–589 (2013)CrossRef
12.
Fiehn, O.: Combining genomics, metabolome analysis, and biochemical modelling to understand metabolic networks. Comp. Funct. Genomics 2, 155–168 (2001)CrossRef
13.
Dunn, W.B., Ellis, D.I.: Metabolomics: current analytical platforms and methodologies. Trend. Anal. Chem. 24, 285–294 (2005)CrossRef
14.
Harborne, J.B.: Phytochemical Methods, 3rd edn. Chapman & Hall, London (1998)
15.
Sumner, L.W., Mendes, P., Dixon, R.A.: Plant metabolomics: large-scale phytochemistry in the functional genomics era. Phytochemistry 62, 817–836 (2003)CrossRef
16.
Xia, J., Mandal, R., Sinelnikov, I.V., Broadhurst, D., Wishart, D.S.: MetaboAnalyst 2.0 - a comprehensive server for metabolomic data analysis. Nucl. Acids Res. 37, W652–W660 (2009)
17.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497 (1962)CrossRef
18.
Wickham, H., Chang, W., RStudio, R Core team: Tools to Make Developing R Packages Easier (2015)
19.
Beleites, C.: Import and Export of Spectra Files. Vignette for the R package hyperSpec (2011)
20.
Bosch Ojeda, C., Sanchez Rojas, F.: Recent developments in derivative ultraviolet/visible absorption spectrophotometry. Anal. Chim. Acta 518, 1–24 (2004)CrossRef
21.
Gamborg, O.L., Murashige, T., Thorpe, T.A., Vasil, I.K.: Plant tissue culture media. In vitro. 12, 473–478 (1976)CrossRef
22.
Rolland, F., Baena-Gonzalez, E., Sheen, J.: Sugar sensing and signaling in plants: conserved and novel mechanisms. Annu. Rev. Plant Biol. 57, 675–709 (2006)CrossRef
23.
Miflin, B.J., Habash, D.Z.: The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops. J. Exp. Bot. 53, 979–987 (2002)CrossRef
24.
Forde, B.G., Lea, P.J.: Glutamate in plants: metabolism, regulation, and signalling. J. Exp. Bot. 58, 2339–2358 (2007)CrossRef
25.
Flores-Samaniego, B., Olivera, H., Gonzalez, A.: Glutamine synthesis is a regulatory signal controlling glucose catabolism in Saccharomyces cerevisiae. J. Bacteriol. 175, 7705–7706 (1993)
26.
DeBerardinis, R.J., Mancuso, A., Daikhin, E., Nissim, I., Yudkoff, M., Wehrli, S., Thompson, C.B.: Beyond aerobic glycolysis: transformed cells can engage in glutamine metabolism that exceeds the requirement for protein and nucleotide synthesis. PNAS 104, 19345–19350 (2007)CrossRef
Metadaten
Titel
UV-Visible Spectrophotometry-Based Metabolomic Analysis of Cedrela Fissilis Velozzo (Meliaceae) Calluses - A Screening Tool for Culture Medium Composition and Cell Metabolic Profiles
verfasst von
Fernanda Kokowicz Pilatti
Christopher Costa
Miguel Rocha
Marcelo Maraschin
Ana Maria Viana
Copyright-Jahr
2015
Buch
9th International Conference on Practical Applications of Computational Biology and Bioinformatics

Print ISBN: 978-3-319-19775-3

Electronic ISBN: 978-3-319-19776-0

Copyright-Jahr: 2015

DOI
https://doi.org/10.1007/978-3-319-19776-0_4