This chapter analyses the fundamental concepts of wildlife and game species, emphasizing their significance in wildlife tourism. Wildlife includes naturally occurring, free-ranging invertebrates and vertebrates, namely animals, fungi, bacteria, and protozoa inhabiting their natural habitats with minimal human influence. Wildlife species are crucial for preserving and maintaining the dynamic equilibrium of their natural habitats by supporting vital ecological processes, including mating, migration, feeding, and symbiosis. Captive individuals of wildlife species in zoological gardens or zoos are rarely considered as part of wildlife, despite lions, elephants, and zebras being the products of free-ranging species. In both protected and unprotected areas, wildlife species autonomously seek ecological resources, including water, food, and shelter, without reliance on humans. While fences and other ecological changes are crucial for species protection, they can sometimes conflict with natural processes and create substantial issues for wildlife habitats. This chapter underscores the essential function of wildlife species in ecosystems, contributing to ecological resilience and wildlife tourism development. It discusses the role of wild animals and plants in the development of wildlife tourism, using Kitulo Plateau National Park as an illustration of a tropical protected area established for the management and conservation of wild flora. The national park is renowned for its seasonal flowering of natural flora, drawing hundreds of nature enthusiasts interested in wildflowers. Such wildlife visitors directly and indirectly help in the preservation of the park for current and future generations. The game species refers to wildlife that may be legally hunted for trophies or valuable components. Various species can be hunted either seasonally or throughout the year for recreational, traditional, and even meat purposes. In short, the game is a subset of wildlife species, and its vocabulary is largely applied to wild animals and not plant life. In many developing countries, wildlife hunting was introduced by the colonial administration during the colonial era. However, ethical and conservation considerations have prevented the killing of some species and terminated entirely tourist hunting, especially in certain wildlife destinations. Various animals, including elephants and black rhinoceroses, are exclusively prohibited by various international and national regulations from hunting business on some hunting grounds. Also, in several nations, such as Kenya, hunting is not a component of wildlife tourism. It is crucial to emphasize that effective biodiversity conservation relies on the balance between social, cultural, economic, and ecological sustainability.