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01-01-2011 | Brief Communication

A new approach to produce amino-carbon nanotubes as plasmid transfection vector by [2 + 1] cycloaddition of nitrenes

Authors: Yongjian Jiang, Chen Jin, Feng Yang, Xianjun Yu, Guojian Wang, Si Cheng, Yang Di, Ji Li, Deliang Fu, Quanxing Ni

Published in: Journal of Nanoparticle Research | Issue 1/2011

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Abstract

Amino-carbon nanotubes (amino-CNTs) can conjugate with the DNA by electrostatic interactions and shuttle the DNA to the cell cytoplasm or even the nucleus. Here we report a new approach to produce amino-CNTs by cycloaddition of nitrenes. Fourier transform infrared spectroscopy was used to verify the success of the functionalization, and the functionalization degree was calculated by thermal gravity analysis. Transmission electron microscope (TEM) was used to observe the solubility of the CNTs and the interactions of the amino-CNTs with the plasmids. Cell ultrathin sections were made and observed under the TEM to confirm the amino-CNTs enter the cells. Transfection experiments ultimately verify the amino-CNTs produced through cycloadditions of nitrenes can serve as plasmid vector.

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Title: A new approach to produce amino-carbon nanotubes as plasmid transfection vector by [2 + 1] cycloaddition of nitrenes
Authors: Yongjian Jiang
Chen Jin
Feng Yang
Xianjun Yu
Guojian Wang
Si Cheng
Yang Di
Ji Li
Deliang Fu
Quanxing Ni
Publication date: 01-01-2011
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 1/2011
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-010-0072-6

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