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Published in:
2015 | OriginalPaper | Chapter
1. Amplification and Sequence Analysis of TPI Gene, a Structural Gene of Operon from Lactobacillus delbrueckii
Authors : T. Pravin Reddy, Dhatrika Sahithi
Published in: Biotechnology and Bioforensics
Publisher: Springer Singapore
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Abstract
The main objective of this work is amplification and sequence analysis of TPI gene which is a structural gene of operon from Lactobacillus delbrueckii. The cow milk was collected under good conditions and analyzed for bacterial load. Many strains of lactic acid bacteria were obtained, out of which 3 strains were homofermentative lactobacilli. Different biochemical tests were done for the identification of Lactobacillus delbrueckii. The TPI gene of Lactobacillus delbrueckii is involved in the maximum production of bacteriocin. Genomic DNA was isolated from Lactobacillus delbrueckii and its TPI gene was successfully amplified using polymerase chain reaction. Further, sequence analysis of TPI gene was done using the bioinformatics tools and the phylogenetic study was made. From the sequence analysis we can infer that the TPI gene sequence of Lactobacillus delbrueckii has close resemblance with the Escherichia coli sequence.