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Published in:
2015 | OriginalPaper | Chapter
2. Analytical Methodology for Lipid Extraction and Quantification from Oleaginous Microorganisms
Authors : Alice Meullemiestre, Cassandra Breil, Maryline Abert-Vian, Farid Chemat
Published in: Modern Techniques and Solvents for the Extraction of Microbial Oils
Publisher: Springer International Publishing
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Abstract
This chapter reviews the development of extraction and analytical techniques for lipids from microorganisms. The commonly used extraction techniques of total lipid such as «Folch», «Bligh and Dyer», and Soxhlet Extraction methods are detailed and explained. A special focus has been made for the complementary analytical methods such as HP-TLC (High Performance Thin Layer Chromatography) which gives details about the repartition of the different classes of lipids and GC (Gas Chromatography) which brings quantitative analysis of all fatty acids chains present in the lipid microbial extract.