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Journal of Crop Health

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25-04-2023 | Original Article / Originalbeitrag

Bioprospecting Biochar and Plant Growth Promoting Rhizobacteria for Alleviating Water Deficit Stress in Vicia faba L.

Authors: Muhammad Nafees, Sami Ullah, Iftikhar Ahmed

Published in: Journal of Crop Health | Issue 6/2023

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Abstract

The aim of the current study was to investigate the drought tolerance of Vicia faba L. using biochar and plant growth-promoting rhizobacteria (PGPR) Sphingobacterium pakistanensis (NCCP246) and Cellulomonas pakistanensis (NCCP11) by analyzing the morpho-physiological attributes. All the plant’s seeds except control were inoculated with PGPRs before being sowed in triplicates in clay pots with 3 kg of soil and sand (2:1) and 5% biochar. Scanning Electron Microscopy (SEM) and Energy Dispersive X‑Ray (EDX) spectroscopy were used to evaluate the morphology and elemental makeup of biochar and leaf surface for trichomes diversity. Element composition, pH, and cation exchange capacity of the soil were significantly improved by biochar. Biochar showed porous structure suitable for water holding and microhabitat provision along with mineral composition analyzed via SEM and EDX. The co-application of PGPR and biochar improved the capacity for drought tolerance as analyzed by studying the leaf morphology, which revealed a considerable decrease in the diversity of trichomes. Antioxidant enzymes including catalase, peroxidase, ascorbate peroxidase, guaiacol peroxidase and dehydroascorbate reductase were significantly boosted up by 44.07–86.58%, 32.06–74.0%, 32.63–61.53%, 31.89–67.24% and 29.41–63.98% respectively. Conclusively, drought mitigation through application of microbes and biochar are metabolic strategies that regulate the activity of antioxidant enzymes, improve plant tolerance by maintaining cellular homeostasis and ameliorate the water potential in agro-ecosystems.

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Title: Bioprospecting Biochar and Plant Growth Promoting Rhizobacteria for Alleviating Water Deficit Stress in Vicia faba L.
Authors: Muhammad Nafees
Sami Ullah
Iftikhar Ahmed
Publication date: 25-04-2023
Publisher: Springer Berlin Heidelberg
Published in: Journal of Crop Health / Issue 6/2023
Print ISSN: 2948-264X
Electronic ISSN: 2948-2658
DOI: https://doi.org/10.1007/s10343-023-00875-8

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