Skip to main content
Disciplines
Automotive Business IT + Informatics Construction + Real Estate Electrical Engineering + Electronics Energy + Sustainability Insurance + Risk Finance + Banking Management + Leadership Marketing + Sales Mechanical Engineering + Materials
Events
DE
EN
Books
Journals
Topic Page
Marketing
Start single access now
Access for companies
EXTENDED SEARCH
Log in
Top
BioControl
Published in: BioControl 6/2018

21-07-2018

Characterization of new Bacillus thuringiensis strains from Iran, based on cytocidal and insecticidal activity, proteomic analysis and gene content

Authors: Ayda Khorramnejad, Reza Talaei-Hassanloui, Vahid Hosseininaveh, Yolanda Bel, Baltasar Escriche

Published in: BioControl | Issue 6/2018

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

Characterization of new Bacillus thuringiensis strains is a valuable tool to discover novel insecticidal toxins and to manage resistance problems. In this study, seven Iranian Bt strains were selected according to their toxicity against Plodia interpunctella, to be thoroughly characterized based on their toxicity, protein profiling, proteomic analysis, gene content and β-exotoxin production. The toxicity was assessed by insect bioassays and cell viability assays (a less cost, time and material consuming technique), using four lepidopteran pests and four lepidopteran cell lines from Trichoplusia ni (Hi5), Helicoverpa zea (HzGUT), Spodoptera exigua (UCR-SE) and Spodoptera frugiperda (Sf21). The selected Bt strains showed similar protein electrophoretic profiles, but differed in toxicity. LC–MS/MS analysis of solubilized crystal proteins and gene content analyses (PCR screening) were compared and correlated with the toxicity results. Based on our data, three Bt strains could be considered as candidates for development of future bioinsecticides.
previous article The effect of cover plants management on soil invertebrate fauna in vineyard in Northern Italy
next article Comparative effects of temperature and thermoregulation on candidate strains of entomopathogenic fungi for Moroccan locust Dociostaurus maroccanus control

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

inform now
Appendix
Available only for authorised users
Literature
Arrieta G, Hernández A, Espinoza AM (2004) Diversity of Bacillus thuringiensis strains isolated from coffee plantations infested with the coffee berry borer Hypothenemus hampei. Rev Biol Trop 52:757–764PubMed
Bel Y, Granero F, Alberola TM, Martínez-Sebastián MJ, Ferré J (1997) Distribution, frequency and diversity of Bacillus thuringiensis in olive tree environments in Spain. Syst Appl Microbiol 20:652–658CrossRef
Berόn CM, Salerno GL (2006) Characterization of Bacillus thuringiensis isolates from Argentina that are potentially useful in insect pest control. BioControl 51:779–794CrossRef
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of dye-binding. Anal Biochem 72:248–254CrossRefPubMed
Bravo A, Likitvivatanavong S, Gill SS, Soberón M (2011) Bacillus thuringiensis: a story of a successful bioinsecticide. Insect Biochem Mol Biol 41:423–431CrossRefPubMedPubMedCentral
Chen Z, He F, Xiao Y, Liu C, Li J, Yang Y, Ai H, Peng J, Hong H, Liu K (2015) Endogenous expression of a Bt toxin receptor in the Cry1Ac-susceptible insect cell line and its synergistic effect with cadherin on cytotoxicity of activated Cry1Ac. Insect Biochem Mol Biol 59:1–17CrossRefPubMed
Djenane Z, Nateche F, Amziane M, Gomis-Cebolla J, El-Aichar F, Khorf H, Ferré J (2017) Assessment of the antimicrobial activity and the entomocidal potential of Bacillus thuringiensis isolates from Algeria. Toxins 9:139–158CrossRefPubMedCentral
Estela A, Escriche B, Ferré J (2004) Interaction of Bacillus thuringiensis toxins with larval midgut binding sites of Helicoverpa armigera (Lepidoptera: Noctuidae). Appl Environ Microbiol 70:1378–1384CrossRefPubMedPubMedCentral
Ferrandis MD, Juárez-Pérez VM, Frutos R, Bel Y, Ferré J (1999) Distribution of cryl, cryll and cryV genes within Bacillus thuringiensis isolates from Spain. Syst Appl Microbiol 22:179–185CrossRef
Ferré J, Escriche B, Bel Y, Van Rie J (1995) Biochemistry and genetics of insect resistance to Bacillus thuringiensis insecticidal crystal proteins. FEMS Microbiol Lett 132:1–7CrossRef
Gelernter WD, Federici BA (1986) Continuous cell line from Spodoptera exigua (Lepidoptera: Noctuidae) that supports replication of nuclear polyhedrosis viruses from Spodoptera exigua and Autographa californica. J Invertebr Pathol 48:199–207CrossRef
Gilliland A, Chambers CE, Bone EJ, Ellar DJ (2002) Role of Bacillus thuringiensis Cry1 δ endotoxin binding in determining potency during lepidopteran larval development. Appl Environ Microbiol 68:1509–1515CrossRefPubMedPubMedCentral
Gomis-Cebolla J, Ruiz de Escudero I, Vera-Velasco NM, Hernández-Martínez P, Hernández-Rodríguez CS, Ceballos T, Palma L, Escriche B, Caballero P, Ferré J (2017) Insecticidal spectrum and mode of action of the Bacillus thuringiensis Vip3Ca insecticidal protein. J Invertebr Pathol 142:60–67CrossRefPubMed
Goodman CL, Wang AA, Nabli H, McIntosh AH, Wittmeyer JL, Grasela JJ (2004) Development and partial characterization of heliothine cell lines from embryonic and differentiated tissues. In Vitro Cell Dev Biol Anim 40:89–94CrossRefPubMed
Granados RR, Li GX, Derksen ACG, McKenna KA (1994) A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus. J Invertebr Pathol 64:260–266CrossRef
Gringorten JL, Sohi SS, Masson L (1999) Activity spectra of Bacillus thuringiensis δ-endotoxins against eight insect cell lines. In Vitro Cell Dev Biol Anim 35:299–303CrossRefPubMed
Hernández CS, Martínez C, Porcar M, Caballero P, Ferré J (2003) Correlation between serovars of Bacillus thuringiensis and type I β-exotoxin production. J Invertebr Pathol 82:57–62CrossRefPubMed
Hernández-Martinez P, Ferré J, Escriche B (2008) Susceptibility of Spodoptera exigua to 9 toxins from Bacillus thuringiensis. J Invertebr Pathol 97:245–250CrossRefPubMed
Hernández-Rodriguez CS, Boets A, van Rie J, Ferré J (2009) Screening and identification of vip genes in Bacillus thuringiensis strains. J Appl Microbiol 107:219–225CrossRefPubMed
Juárez-Pérez VM, Ferrandis MD, Frutos R (1997) PCR-based approach for detection of novel Bacillus thuringiensis cry gene. Appl Environ Microbiol 63(8):2997–3002PubMedPubMedCentral
Kwa MSG, de Maagd RA, Stiekema WJ, Vlak JM, Bosch D (1998) Toxicity and binding properties of the Bacillus thuringiensis delta-endotoxin Cry1C to cultured insect cells. J Invertebr Pathol 71:121–127CrossRefPubMed
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefPubMed
McGaughey WH, Beeman RW (1988) Resistance to Bacillus thuringiensis in colonies of Indian meal moth and almond moth (Lepidoptera: Pyralidae). J Econ Entomol 81:28–33CrossRef
Ohba M, Mizuki E, Uemori A (2009) Parasporin, a new anticancer protein group from Bacillus thuringiensis. Anticancer Res 29:427–433PubMed
Porcar M, Juárez-Pérez VM (2003) PCR-based identification of Bacillus thuringiensis pesticidal crystal genes. FEMS Microbiol Rev 26:419–432CrossRefPubMed
Salehi Jouzani G, AbadAP Seifinejad A, Marzban R, Kariman K, Maleki B (2008) Distribution and diversity of dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran. J Ind Microbiol Biotechnol 35:83–94CrossRef
Scaramal Ricietto AN, Gomis-Cebolla J, Vilas-Boas GT, Ferré J (2016) Susceptibility of Grapholita molesta (Busck, 1916) to formulations of Bacillus thuringiensis, individual toxins and their mixtures. J Invertebr Pathol 141:1–5CrossRef
Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev 62:775–806PubMedPubMedCentral
Seifinejad A, Salehi Jouzani G, Hosseinzadeh A, Abdmishani C (2008) Characterization of Lepidoptera-active cry and vip genes in Iranian Bacillus thuringiensis strain collection. Biol Control 44:216–226CrossRef
Shojaaddini M, López MJ, Moharramipour S, Khodabandeh M, Talebi AA, Vilanova C, Latorre A, Porcar M (2012) A Bacillus thuringiensis strain producing epizootics on Plodia interpunctella: a case study. J Stored Prod Res 48:52–60CrossRef
Siebert MW, Nolting S, Leonard BR, Braxton LB, All JN, van Duyn JW, Bradley JR, Bacheler J, Huckaba RM (2008) Efficacy of transgenic cotton expressing Cry1Ac and Cry1F insecticidal protein against heliothines (Lepidoptera: Noctuidae). J Econ Entomol 101:1950–1959CrossRefPubMed
Thammasittirong A, Attathom T (2008) PCR-based method for the detection of cry genes in local isolates of Bacillus thuringiensis from Thailand. J Invertebr Pathol 98:121–126CrossRefPubMed
Uribe D, Martinez W, Ceron J (2003) Distribution and diversity of cry genes in native strains of Bacillus thuringiensis obtained from different ecosystems from Colombia. J Invertebr Pathol 82:119–127CrossRefPubMed
van Frankenhuyzen K (2009) Insecticidal activity of Bacillus thuringiensis crystal proteins. J Invertebr Pathol 101:1–16CrossRefPubMed
Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P (1977) The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13:213–217CrossRefPubMed
Wang JH, Boets A, Van Rie J, Ren GX (2003) Characterization of cry1, cry2 and cry9 genes in Bacillus thuringiensis isolated from China. J Invertebr Pathol 82:63–71CrossRefPubMed
Willcoxon MI, Dennis JR, Lau SI, Xie W, You Y, Leng S, Fong RC, Yamamoto T (2016) A high-throughput, in-vitro assay for Bacillus thuringiensis insecticidal proteins. J Biotechnol 217:72–81CrossRef
Witt DP, Carson H, Hodgdon JC (1986) Cytotoxicity of Bacillus thuringiensis δ-endotoxins to cultured Cf-1 cells does not correlate with in vivo activity towards spruce budworm larvae. In: Samson RA, Vlak JM, Peters D (eds) Fundamental and applied aspects of invertebrate pathology. Academic Press, San Diego, pp 3–6
Zhao C, Jurat-Fuentes JL, Abdelgaffar HM, Pan H, Song F, Zhang J (2015) Identification of new cry1I-type gene as a candidate for gene pyramiding in corn to control Ostrinia species larvae. Appl Environ Microbiol 81:3699–3705CrossRefPubMedPubMedCentral
Metadata
Title
Characterization of new Bacillus thuringiensis strains from Iran, based on cytocidal and insecticidal activity, proteomic analysis and gene content
Authors
Ayda Khorramnejad
Reza Talaei-Hassanloui
Vahid Hosseininaveh
Yolanda Bel
Baltasar Escriche
Publication date
21-07-2018
Publisher
Springer Netherlands
Published in
BioControl / Issue 6/2018
Print ISSN: 1386-6141
Electronic ISSN: 1573-8248
DOI
https://doi.org/10.1007/s10526-018-9901-9

Other articles of this Issue 6/2018

BioControl 6/2018 Go to the issue

OriginalPaper

Effect of host feeding on life history traits of Tamarixia radiata, parasitoid of the Asian citrus psyllid, Diaphorina citri

OriginalPaper

Factors influencing the dispersal of a native parasitoid, Phasgonophora sulcata, attacking the emerald ash borer: implications for biological control

OriginalPaper

The effect of cover plants management on soil invertebrate fauna in vineyard in Northern Italy

OriginalPaper

Standardization of prey immunomarking: does a positive test always indicate predation?

OriginalPaper

Rate of consumption, biological parameters, and population growth capacity of Orius laevigatus fed on Spodoptera exigua

OriginalPaper

Comparative effects of temperature and thermoregulation on candidate strains of entomopathogenic fungi for Moroccan locust Dociostaurus maroccanus control