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Environmental Earth Sciences

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01-12-2023 | Original Article

Chemico-mechanical activation of EICP-modified soil: role of urease enzyme

Authors: Pranamee Baruah, Susmita Sharma

Published in: Environmental Earth Sciences | Issue 23/2023

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Abstract

Enzyme-induced carbonate precipitation (EICP) is a ground improvement technique used in bio-geotechnical engineering, which utilizes environmental-friendly and favorable components such as urea, calcium chloride, and activator enzyme for soil modification. Utilization of such combined treatment techniques is site specific. In this regard, research was conducted to investigate the effectiveness of varied concentrations of EICP cementing solution on locally collected natural soil, with a special emphasis on the protocol of modification. The effect of curing time on strength development of soils stabilized with varying proportions of urea, calcium chloride, and urease enzyme was investigated, subjected to three cycles of treatment process. The protocol followed for soil-less experiments undertaken with varying proportions of cementing solution helped to determine the maximum carbonate (CaCO₃) precipitation in the cemented soils. A combination of 0.5 M urea, 1 M CaCl_2, and 6 g/L of urease enzyme is proposed as the cementing media to investigate the increase in unconfined compressive strength (UCS) of the treated soils. The ratio of CaCO₃ precipitation as a function of the number of treatment cycles is also investigated. The results highlighted that treatment cycles are a responsible parameter for increased precipitation and higher UCS (~ up to 76 kPa and 90.62 kPa) in the natural soils. The qualitative assessment of the precipitates done by FE-SEM micrographs XRD analysis and, revealed heterogeneity in the distribution of Ca in the cemented soil microenvironment.

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Title: Chemico-mechanical activation of EICP-modified soil: role of urease enzyme
Authors: Pranamee Baruah
Susmita Sharma
Publication date: 01-12-2023
Publisher: Springer Berlin Heidelberg
Published in: Environmental Earth Sciences / Issue 23/2023
Print ISSN: 1866-6280
Electronic ISSN: 1866-6299
DOI: https://doi.org/10.1007/s12665-023-11255-w

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