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2013 | OriginalPaper | Chapter
Detection of N-Acetyl-D-Glucosamine in Hyaluronan by Thin Layer Chromatography
Authors : Nguyen H. K. Tu, Pham V. M. Thien
Published in: 4th International Conference on Biomedical Engineering in Vietnam
Publisher: Springer Berlin Heidelberg
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Various reactions that produce color are known to be selective for certain types of carbohydrates. It was difficult to identify N-acetyl-glucosamine in hyaluronan because the hyaluronan was produced very low in microorgansims. Besides, in the traditional methods of Morgan-Elson, using alkali borate to lysis hyaluronan to give N-acety-D-glucosamine caused the unspecific products that could not react to di-methyl-benzaldehyde (DMAB). The study developed the method to detect N-acetyl-D-glucosamine by thin layer chromatography (TLC) that showed the stable purple traces on TLC than in liquid. The study was confirmed by applying high pressure liquid chromatography (HPLC).