Development of ready-to-use products derived from Bacillus subtilis strain CMs026 for plant disease control

Bacillus subtilis CMs026 is used for plant disease control. However, use of biocontrol for crops is limited by variable performances of control agents. Therefore, this study aimed to develop a ready-to-use, bacteria-based, highly stable, solid dosage formulation for plant disease control. Incubated broths containing B. subtilis CMs026 were centrifuged and sterilized. Supernatants were mixed with silicon dioxides and dried at 80 °C for 48 h. Agar diffusion test using active antifungal substance, surfactin, as a marker revealed the efficacy of dried broth. Subsequently, products were prepared by mixing dried broths with 6% disintegrant, followed by compaction. The compressed tablets were stable after storage for three months. In vivo experiments against Alternaria brassicicola in Chinese kale (Brassica alboglabra) revealed that the products reduced percent crop loss from 88% to 40%.