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02-03-2020

Efficacy of a native isolate of the entomopathogenic fungus Metarhizium anisopliae against larval tick outbreaks under semifield conditions

Authors: Emily Mesquita, Allan F. Marciano, Amanda R. C. Corval, Jéssica Fiorotti, Thaís A. Corrêa, Simone Quinelato, Vânia R. E. P. Bittencourt, Patrícia S. Golo

Published in: BioControl | Issue 3/2020

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Abstract

This study aimed to evaluate Metarhizium anisopliae (Metschn.) Sorokin (Hypocreales: Clavicipitaceae) persistence in the soil and its impact on Rhipicephalus microplus Canestrini (Acari: Ixodidae) larval recovery in a semifield trial after the treatment of female ticks. Nine strains from the genus Metarhizium Sorokin were isolated from the soil in Brazil and taxonomically classified using the ef1-α gene. The thermotolerance of the strains and their in vitro virulence to tick larvae were tested. One M. anisopliae strain was selected and formulated for the semifield test. The presence of M. anisopliae in the soil ranged from 0.4 × 10⁵ to 1.4 × 10⁵ colony forming units per gram of soil after the treatment during the five months of the survey. The fungus-treated grass pots had significantly fewer larvae than did the control pots. Evidence was gathered about the soil persistence of a native M. anisopliae strain and its efficacy in the biological control of ticks.

previous article Efficacy of Metarhizium anisopliae conidia in oil-in-water emulsion against the tick Rhipicephalus microplus under heat and dry conditions

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Title: Efficacy of a native isolate of the entomopathogenic fungus Metarhizium anisopliae against larval tick outbreaks under semifield conditions
Authors: Emily Mesquita
Allan F. Marciano
Amanda R. C. Corval
Jéssica Fiorotti
Thaís A. Corrêa
Simone Quinelato
Vânia R. E. P. Bittencourt
Patrícia S. Golo
Publication date: 02-03-2020
Publisher: Springer Netherlands
Published in: BioControl / Issue 3/2020
Print ISSN: 1386-6141
Electronic ISSN: 1573-8248
DOI: https://doi.org/10.1007/s10526-020-10006-1

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