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Medical & Biological Engineering & Computing

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01-05-2016 | Original Article

Estimation of dynamic metabolic activity in micro-tissue cultures from sensor recordings with an FEM model

Authors: Cornelia Pfister, Christian Forstmeier, Johannes Biedermann, Julia Schermuly, Franz Demmel, Peter Wolf, Bernd Kaspers, Martin Brischwein

Published in: Medical & Biological Engineering & Computing | Issue 5/2016

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Abstract

We estimated the dynamic cell metabolic activity and the distribution of the pH value and oxygen concentration in tissue samples cultured in vitro by using real-time sensor records and a numerical simulation of the underlying reaction–diffusion processes. As an experimental tissue model, we used chicken spleen slices. A finite element method model representing the biochemical processes and including the relevant sensor data was set up. By fitting the calculated results to the measured data, we derived the spatiotemporal values of the pH value, the oxygen concentration and the absolute metabolic activity (extracellular acidification and oxygen uptake rate) of the samples. Notably, the location of the samples in relation to the sensors has a great influence on the detectable metabolic rates. The long-term vitality of the tissue samples strongly depends on their size. We further discuss the benefits and limitations of the model.

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Title: Estimation of dynamic metabolic activity in micro-tissue cultures from sensor recordings with an FEM model
Authors: Cornelia Pfister
Christian Forstmeier
Johannes Biedermann
Julia Schermuly
Franz Demmel
Peter Wolf
Bernd Kaspers
Martin Brischwein
Publication date: 01-05-2016
Publisher: Springer Berlin Heidelberg
Published in: Medical & Biological Engineering & Computing / Issue 5/2016
Print ISSN: 0140-0118
Electronic ISSN: 1741-0444
DOI: https://doi.org/10.1007/s11517-015-1367-7

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