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Published in:
2018 | OriginalPaper | Chapter
10. Experimental Methods
Author : Amandeep Kaur
Published in: Fluorescent Tools for Imaging Oxidative Stress in Biology
Publisher: Springer International Publishing
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Abstract
\(^{1}\)H NMR spectra were obtained at 300 K using Bruker DRX 300 or Bruker Ascend 400 and 500 spectrometers at frequencies of 300, 400 and 500 MHz, respectively. \(^{13}\)C NMR spectra were recorded on these spectrometers at frequencies of 75, 100, and 125 MHz. Deuterated solvents were obtained from Cambridge Isotope Laboratories were used, and internal references were made using the residual solvent peak. The \(^{1}\)H NMR data are reported as chemical shift (\(\delta \)) in ppm, and coupling constant (J) in Hz. The \(^{13}\)C NMR data are reported as chemical shift (\(\delta \)) in ppm downfield shift.