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Introduction to Hydrogels Read chapter Read first chapter

2010 | OriginalPaper | Chapter

Gelatin-Based Hydrogels for Controlled Cell Assembly

Authors : Xiaohong Wang, Yongnian Yan, Renji Zhang

Published in: Biomedical Applications of Hydrogels Handbook

Publisher: Springer New York

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Abstract

Controlled cell assembly technique is a new research area in complex organ development technologies. Gelatin-based hydrogels, such as gelatin, gelatin/alginate, gelatin/chitosan, gelatin/fibrinogen, gelatin/hyaluronan, and gelatin/alginate/fibrinogen, have played an important role in the rapid fabrication of tissue or organs with well-defined structures and functions. Cryoprotectants, such as dimethylsulfoxide (DMSO) and glycerol, can be easily incorporated into the system for long-term conservation of the cell containing constructs. Hepatocytes, chondrocytes, cardiac myocytes, and adipose-derived stromal cells (ADSCs) are used to show function of the assembled cells. ADSCs can be controlled to differentiate into different targeted cell types according to their positions within the orderly predesigned three-dimensional (3D) constructs. A multicellular model for the metabolic syndrome was established along with the development of the double-syringe deposition system which lead to a hybrid cell/hydrogels construct with a vascular-like network fabricated using a digital model. The preliminary results indicate that the double-syringe assembly technique is a powerful tool for fabricating complex constructs with special intrinsic/extrinsic structures, and has the potential to be widely used in regenerative medicine and drug screening.

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previous chapter Hydrogels for Cartilage Tissue Engineering
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Metadata
Title
Gelatin-Based Hydrogels for Controlled Cell Assembly
Authors
Xiaohong Wang
Yongnian Yan
Renji Zhang
Copyright Year
2010
Publisher
Springer New York
Book
Biomedical Applications of Hydrogels Handbook

Print ISBN: 978-1-4419-5918-8

Electronic ISBN: 978-1-4419-5919-5

Copyright Year: 2010

DOI
https://doi.org/10.1007/978-1-4419-5919-5_14

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